Von Willebrand Factor Human ELISA Kit (ab108918)
Overview
- Product nameVon Willebrand Factor Human ELISA KitSee all Von Willebrand Factor kits ...
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich
- Sensitivity= 2.5 mU/ml
- Range2.5 mU/ml - 80 mU/ml
- Recovery
100.5 %
- Assay time5h 00m
- Species reactivityReacts with: Human
- Product overview
Abcam’s Von Willebrand Factor (VWF) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Human VWF in serum, plasma and cell culture supernatants.
Ab108918 VWF ELISA kit is designed for detection of human VWF in plasma, serum and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures VWF in less than 5 hours. A murine antibody specific for VWF has been pre-coated onto a microplate. Human VWF in standards and samples is sandwiched by the immobilized monoclonal antibody and biotinylated polyclonal antibody specific for VWF, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
- Notes
Von Willebrand factor (VWF) is a multimeric glycoprotein that circulates in blood forming a noncovalent complex with procoagulant factor VIII. During normal homeostasis, the larger multimers of VWF are responsible for facilitating platelet plug formation by forming a bridge between platelet glycoprotein IB and exposed collagen in the subendothelium. The congenital dysfunctional state of VWF causes a moderate to severe bleeding diathesis-von Willebrand disease (VWD).
- Tested applicationsSandwich ELISA more details
Properties
- Storage instructionsStore at +4°C. Please refer to protocols.
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Components 1 x 96 tests Biotinylated VWF Antibody (80x) 1 x 100µl Chromogen Substrate 1 x 8ml Diluent Concentrate (10x) 1 x 30ml Stop Solution 1 x 12ml Streptavidin-Peroxidase Conjugate (SP Conjugate) 1 x 80µl VWF Microplate 1 unit VWF Standard 1 unit Wash Buffer Concentrate (20x) 1 x 30ml - Research Areas
Defects in VWF are the cause of von Willebrand disease type 2 (VWD2) [MIM:613554]. A hemorrhagic disorder due to defects in von Willebrand factor protein and resulting in impaired platelet aggregation. Von Willebrand disease type 2 is characterized by qualitative deficiency and functional anomalies of von Willebrand factor. It is divided in different subtypes including 2A, 2B, 2M and 2N (Normandy variant). The mutant VWF protein in types 2A, 2B and 2M are defective in their platelet-dependent function, whereas the mutant protein in type 2N is defective in its ability to bind factor VIII. Clinical manifestations are mucocutaneous bleeding, such as epistaxis and menorrhagia, and prolonged bleeding after surgery or trauma.
Defects in VWF are the cause of von Willebrand disease type 3 (VWD3) [MIM:277480]. A severe hemorrhagic disorder due to a total or near total absence of von Willebrand factor in the plasma and cellular compartments, also leading to a profound deficiency of plasmatic factor VIII. Bleeding usually starts in infancy and can include epistaxis, recurrent mucocutaneous bleeding, excessive bleeding after minor trauma, and hemarthroses.
Contains 4 TIL (trypsin inhibitory-like) domains.
Contains 3 VWFA domains.
Contains 3 VWFC domains.
Contains 4 VWFD domains.
modificationsAll cysteine residues are involved in intrachain or interchain disulfide bonds.
N- and O-glycosylated.
Target information above from: UniProt accession
P04275
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- Coagulation factor VIIIcoagulation factor VIII VWFF8VWF
- F8VWFFactor VIII related antigenFACTOR VIII-VON WILLEBRAND FACTORvon Willebrand antigen 2Von Willebrand antigen IIVon Willebrand diseasevon Willebrand factorVWDVWDVWFVWF_HUMAN
see all
- Entrez Gene: 7450 Human
- Omim: 613160 Human
- SwissProt: P04275 Human
- Unigene: 440848 Human
Applications
Our Abpromise guarantee covers the use of ab108918 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Sandwich ELISA | sELISA |
Von Willebrand Factor Human ELISA Kit images
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ELISA - Von Willebrand Factor Human ELISA Kit (ab108918)TYPICAL STANDARD CURVE – Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
Protocols
References for Von Willebrand Factor Human ELISA Kit (ab108918)
ab108918 has not yet been referenced specifically in any publications.
