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suche AK gegen VWF gegen D3-Domaene (=Bdgsstelle fuer Factor VIII; d.h. zum Blocken der Bdg). ELISA/Biacore, human |
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ANSWER: |
Vielen Dank für Ihren Anruf. |
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Bonsoir, Pouvez-vous me renseigner sur le vWF anti humain ab20435 à propos de son application en cytométrie Faut-il pour l’utiliser perméabiliser la membrane des cellules endothéliales ? Avez-vous un protocole associé ? Merci de bien vouloir m’apporter votre réponse Cordialement
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ANSWER: |
Merci de nous avoir contactés.
Le laboratoire m'a informé que l'anticorps ab20435 a été utilisé en cytométrie en flux sur cellules Jurkat et thrombocytes.
- Cellules Jurkat : fixation avec 1% formaldéhyde et perméabilisation avec une solution contenant de la saponine.
- Thrombocytes : fixation avec 1% formaldéhyde, rinçage et suspension dans du PBS sans perméabilisation.
J'espère que ces informations vous seront utiles. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions. |
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When this product was tested for flow cytometry, was it tested on HUVECs collected using trypsin? Was a permeabilization step performed prior to staining? Finally, did FITC and PE secondary antibodies work equally well with this primary antibody. Any information available will be helpful. |
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ANSWER: |
Thank you for your enqiry regarding the FACS testing of this antibody (ab20435). The flow cytometry application for this antibody is in a published reference. The full text is available free on-line at http://www.bloodjournal.org/cgi/reprint/91/2/491.pdf The sample preparation and flow cytometry protocol used can be found in the Materials and Methods on Page 492. Testing in this procedure was done after FITC-labeling of the antibody. I hope this helps! Please don't hesitate to get in touch again if you have any more questions. With thanks |
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