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Products:Cardiovascular >> Blood >> Serum Proteins
Overview
Product name
Anti-Von Willebrand Factor antibody [4F9]
See all Von Willebrand Factor products (23) ...
Description
Mouse monoclonal [4F9] to Von Willebrand Factor
Tested applications
IF, Flow Cyt, IHC-Frmore details
Cross reactivity
Reacts with
Human
Immunogen
FVIII: C/vWF complex (denaturated)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS, 1mg/ml BSA
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Clone number
4F9
Isotype
IgG1
Light chain type
kappa
Research areas
Stem Cells >> Endothelial Progenitors >> Endothelial Markers
Cancer >> Invasion/microenvironment >> Angiogenesis >> Angiogenic growth factors
Cardiovascular >> Blood >> Coagulation >> Regulatory
Cardiovascular >> Blood >> Coagulation >> Extrinsic
Cardiovascular >> Blood >> Platelets
Cardiovascular >> Blood >> Serum Proteins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab20435 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IF: Use 5µg for 105 cells.
Flow Cyt: Use 2µg for 105 cells. (. ab20435 has been used in flow cytometry with Jurkat cells and with platelets. The Jurkat cell line was fixed using 1% formaldehyde followed by permeabilization using a commercially-available saponin reagent for permeabilization. The platelets were fixed using 1% formaldehyde, washed and suspended in PBS without permeabilization.)
IHC-Fr
IHC-Fr: 1/50 - 1/100.IHC-Fr: 1/50 - 1/100.
Target
Function
Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma.
Tissue specificity
Plasma.
Involvement in disease
Defects in VWF are the cause of von Willebrand disease (VWD) [MIM:277480]. VWD defines a group of hemorrhagic disorders in which the von Willebrand factor is either quantitatively or qualitatively abnormal resulting in altered platelet function. Symptoms vary depending on severity and disease type but may include prolonged bleeding time, deficiency of factor VIII and impaired platelet adhesion. Type I von Willebrand disease is the most common form and is characterized by partial quantitative plasmatic deficiency of an otherwise structurally and functionally normal Willebrand factor; type II is associated with a qualitative deficiency and functional anomalies of the Willebrand factor; type III is the most severe form and is characterized by total or near-total absence of Willebrand factor in the plasma and cellular compartments, also leading to a profound deficiency of plasmatic factor VIII.
Sequence similarities
Contains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 4 TIL (trypsin inhibitory-like) domains.
Contains 3 VWFA domains.
Contains 3 VWFC domains.
Contains 4 VWFD domains.
Domain
The von Willebrand antigen 2 is required for multimerization of vWF and for its targeting to storage granules.
Post-translational
modifications
All cysteine residues are involved in intrachain or interchain disulfide bonds.
N- and O-glycosylated.
Cellular localization
Secreted. Secreted > extracellular space > extracellular matrix. Localized to storage granules.
Target information above from: UniProt accessionP04275
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Coagulation factor VIII antibody
- F8VWF antibody
- Factor VIII related antigen antibody
see all
Database links
- Entrez Gene: 7450 Human
- Omim: 193400 Human
- SwissProt: P04275 Human
- Unigene: 440848 Human
References for Anti-Von Willebrand Factor antibody [4F9] (ab20435)
ab20435 has not yet been referenced specifically in any publications.
Publishing research using ab20435? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"