Von Willebrand Factor antibody [F8/86] (ab778)

Product Name 

Von Willebrand Factor antibody [F8/86]

Product type 

Primary antibodies

Description 

Mouse monoclonal [F8/86] to Von Willebrand Factor

Immunogen 

Von Willebrand Factor isolated from human plasma.

Reacts with 
(species key)

Hu, Rb

Specificity 

This antibody reacts with Von Willebrand Factor in endothelial cells.
It also reacts with megakaryocytes in human bone marrow.

Tested applications 
(see key)

IHC-Fr, IHC-P

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
IHC-P: 1/15 - 1/30. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
IHC-Fr: 1/15 - 1/30.
This antibody may be diluted to a titer of 1:15-1:30 in an ABC method. Staining Protocol: We suggest an incubation period of 60 minutes at room temperature.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Secreted. Localized to storage granules.

Research areas 

Stem Cells >> Endothelial Progenitors >> Endothelial Markers
Cardiovascular >> Blood >> Coagulation >> Regulatory
Cardiovascular >> Blood >> Coagulation >> Extrinsic
Cardiovascular >> Blood >> Platelets
Cardiovascular >> Blood >> Serum Proteins

Relevance 

Von Willebrand Factor (VWF) was previously known as Factor VIII related antigen. VWF is synthesized exclusively by endothelial cells and megakaryocytes, and stored in the intracellular granules or constitutively secreted into plasma. This glycoprotein functions as both an antihemophilic factor carrier and a platelet vessel wall mediator in the blood coagulation system. Important in the maintenance of homeostasis, it participates in platelet vessel wall interactions by forming a noncovalent complex with coagulation factor VIII at the site of vascular injury. The Von Willebrand factor has functional binding domains to platelet glycoprotein Ib, glycoprotein IIb/IIIa, collagen and heparin. Mutations in this gene or deficiencies in this protein result in Von Willebrand's disease. VWD is characterized by frequent bleeding (gingival, minor skin quantitative lacerations, menorrhagia, etc.).

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

F8/86

Isotype 

IgG1

Light chain type 

kappa

Purity 

Tissue culture supernatant

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 1% BSA, Tissue culture supernatant

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.150 mg/ml

Storage instructions 

Store at +4°C.

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References for Von Willebrand Factor antibody [F8/86] (ab778)

  This product has been used in: (two most recent references)

Chen X et al. Caffeine blocks disruption of blood brain barrier in a rabbit model of Alzheimer's disease. J Neuroinflammation 5:12 (2008). IHC-Fr; Rabbit. PubMed: 18387175

Naiem M et al. The value of immunohistological screening in the production of monoclonal antibodies. J Immunol Methods 50:145-60 (1982). PubMed: 6806388
If you publish research using ab778 please let us know so that we can cite the reference on this datasheet.

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