Anti-Von Willebrand Factor antibody (FITC) (ab8822)

Product code: 8822
Lot number: GR36100-5

Inquiry: To evaluate the purity of rat sinusoidal endothelial cells(rLSEC), human umbilical vein cells(HUVEC, as positive control) and rat hepatic stellate cell line(HSC-T6, as negative control) were chose. In result, however, the HSC-T6 was also stained by vWf. Thank you.

ANSWER:

Thank you for your message.

I am sorry I am not sure what you require from us. I would appreciate if you are able to provide further clarification and more information.

Are you having difficulty obtaining results from ab8822 Von Willebrand Factor antibody? In which caseam sorry tohear this andwe would be pleased to investigate.Are you obtaining positive staining inwhat would be the expectednegative control?

In this case, i would appreciate if you are able to provide the following information:

1. Which application you are using, was this ChIP?And the protocol you are using?

2. Any images which would helpful forus to assess the results. From both the positive control and negative control samples.

3. The order number and date of purchase.

Many thanks for your time and cooperation. I look forward to hearing from you.

I would like to test ab8822 for use on mouse samples.

ANSWER:

DISCOUNT CODE: ***
Expiration date: September 11th, 2012

I am very pleased to hear you would like to accept our offer and test ab8822 in mouse. This code will give you: 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for mouse and include this code in the "Additional Comments" section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: http://www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: http://www.abcam.com/collaborationdiscount.

would the isotype control (sheep IgG, FITC-conjugated) be available for sale?

ANSWER:

Thank you for your patience.

I am checking with the supplying lab, but it might well be that they do not have such isotype control.

As an alternative, an unconjugated isotype control could be used together with a secondary antibody. For instance:

ab37385 - Sheep IgG - Isotype Control (http://www.abcam.com/Sheep-IgG-Isotype-Control-ab37385.html)

with FITC-conjugated secondary antibody ab7107 - Rabbit polyclonal Secondary Antibody to Sheep IgG - H&L (FITC), pre-adsorbed (http://www.abcam.com/Sheep-IgG-secondary-antibody-H-L-Pre-Adsorbed-ab7107.html)

As soon as I receive more information from the supplying lab, I will let you know.

I hope this information is nevertheless helpful to you. Please do not hesitate to contact us if you need any more advice or information.

1) Looking for a positive control for ab8822, an anti-Von Willebrand Factor antibody (FITC).

2) a quotation for 1unit of ab8822

ANSWER:

Merci de nous avoir contactés.

J'ai regardé de plus près les fiches techniques des autres anti-Von Willebrand Factor. Les liens ci-dessous vous seront peut-être utiles :

exemple de marquage ICC/IF sur cellules endotheliales microvasculaire coronaire HMVEC-c : http://www.abcam.com/index.html?datasheet=6994&tab=abreviews&intabreviewid=21132 .

exemple de marquage ICC/IF sur cellules PMN : http://www.abcam.com/Von-Willebrand-Factor-antibody-ab9378.html#Von-Willebrand-Factor-Primary-antibodies-ab9378-6.jpg .

Lien vers la liste des tissus et le niveau d'expression de Von Willebrand Factor : http://www.ncbi.nlm.nih.gov/UniGene/ESTProfileViewer.cgi?uglist=Hs.440848

Pour l'édition du devis, pouvez-vous m'envoyer s'il vous plait les coordonnées de votre laboratoire?

J'espère que ces informations vous seront utiles. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

Thanks for your help on this matter. Much appreciated. 

I have attached an email showing the purchase of this anti lacZ antibody from March 24 2011... just short of 6 months. 

The current lot # we are using is 134764. Would you happen to know if there is a different lot to try?  

Also I was hoping to just place the order through Cedarlane again if that is OK with you? 

Thanks again

ANSWER:

Thank you for your email.

Unfortunately we only have the same lot in stock, would you like a replacement from the same lot or an alternative product? We will be able to organise the replacement with Cedarlane.

Looking forward to your reply.