Von Willebrand Factor antibody (FITC) (ab8822)

Product Name 

Von Willebrand Factor antibody (FITC)

Product type 

Primary antibodies

Description 

Sheep polyclonal to Von Willebrand Factor (FITC)

Immunogen 

Human Von Willebrand Factor Antigen prepared from citrated human plasma.
Greater than 95% purity by SDS-PAGE.

Reacts with 
(species key)

Hu

Specificity 

The unconjugated immunoglobulin gives no arcs when tested by immunoelectrophoresis against human serum.
Identity has been confirmed by double diffusion (Ouchterlony) against von Willebrand Factor and an anti-von Willebrand Factor of known specificity.

Tested applications 
(see key)

IHC-Fr

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
IHC-Fr: 1/50 - 1/100.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Direct on frozen sections of human tonsil.

Cellular localization 

Secreted. Localized to storage granules.

Research areas 

Stem Cells >> Endothelial Progenitors >> Endothelial Markers
Cardiovascular >> Blood >> Coagulation >> Regulatory
Cardiovascular >> Blood >> Coagulation >> Extrinsic
Cardiovascular >> Blood >> Platelets
Cardiovascular >> Blood >> Serum Proteins

Relevance 

Von Willebrand Factor (VWF) was previously known as Factor VIII related antigen. VWF is synthesized exclusively by endothelial cells and megakaryocytes, and stored in the intracellular granules or constitutively secreted into plasma. This glycoprotein functions as both an antihemophilic factor carrier and a platelet vessel wall mediator in the blood coagulation system. Important in the maintenance of homeostasis, it participates in platelet vessel wall interactions by forming a noncovalent complex with coagulation factor VIII at the site of vascular injury. The Von Willebrand factor has functional binding domains to platelet glycoprotein Ib, glycoprotein IIb/IIIa, collagen and heparin. Mutations in this gene or deficiencies in this protein result in Von Willebrand's disease. VWD is characterized by frequent bleeding (gingival, minor skin quantitative lacerations, menorrhagia, etc.).

Database links 

Raised in 

Sheep

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Conjugation notes 

Fluorescein Isothiocyante (FITC)

Storage buffer 

PBS, pH 7.2, sodium azide, 0.1%

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Conjugation 

Fluorescein

Concentration 

10.000 mg/ml

Storage instructions 

Store at +4°C.

References for Von Willebrand Factor antibody (FITC) (ab8822)

  This product has been used in:

Wang W et al. Netrin-1 and kidney injury. I. Netrin-1 protects against ischemia-reperfusion injury of the kidney. Am J Physiol Renal Physiol 294:F739-47 (2008). PubMed: 18216145

If you publish research using ab8822 please let us know so that we can cite the reference on this datasheet.

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