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Immunocytochemistry/ Immunofluorescence - Von Willebrand Factor antibody (ab6994)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Von Willebrand Factor antibody (ab6994)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Von Willebrand Factor antibody (ab6994)
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Product Name
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Von Willebrand Factor antibody
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See all Von Willebrand Factor antibodies (24)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Von Willebrand Factor
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Immunogen
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Full length native protein (purified from plasma) (Human).
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Reacts with
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Hu, Rat, Gpig
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Does not react with
(species key)
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Chk
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Specificity
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The product specifically reacts with the cytoplasm of human endothelial cells from normal and neoplastic blood and from lymphatic vessels. It also reacts with human endocardium, platelets and megakaryocytes.
Historically, this antibody worked well in mouse (see references), however more recent batches have shown inconsistent results in mouse. Therefore, we do not recommend this antibody for use in this species.
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Tested applications
(see key)
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ICC/IF, IHC-FoFr, IHC-Fr, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: 1/400.
IHC-P: 1/200 - 1/400 for IF and 1/1000-1/2000 for ABC methods with HRP conjugates. Perform enzymatic antigen retrieval with 0.1% pronase for 10 min at 35 °C before commencing with IHC staining protocol.
IHC-Fr: 1/1000.
IHC-FoFr: Use at an assay dependent dilution (PMID 19622235).
Indirect Immunofluorescence: a minimum working dilution of 1:200 was determined using formalin-fixed, paraffin-embedded sections of human tongue using a FITC-conjugated secondary antibody.
Indirect Immunoperoxidase Labeling: a minimum working dilution of 1:800 was determined using formalin-fixed, paraffin-embedded sections of human tongue using a biotinylated secondary antibody and signal amplification.
WB: 1/500.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human tongue.
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Cellular localization
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Secreted. Localized to storage granules.
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Research areas
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Stem Cells >> Endothelial Progenitors >> Endothelial Markers
Cardiovascular >> Blood >> Coagulation >> Regulatory
Cardiovascular >> Blood >> Coagulation >> Extrinsic
Cardiovascular >> Blood >> Platelets
Cardiovascular >> Blood >> Serum Proteins
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Relevance
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Von Willebrand Factor (VWF) was previously known as Factor VIII related antigen. VWF is synthesized exclusively by endothelial cells and megakaryocytes, and stored in the intracellular granules or constitutively secreted into plasma. This glycoprotein functions as both an antihemophilic factor carrier and a platelet vessel wall mediator in the blood coagulation system. Important in the maintenance of homeostasis, it participates in platelet vessel wall interactions by forming a noncovalent complex with coagulation factor VIII at the site of vascular injury. The Von Willebrand factor has functional binding domains to platelet glycoprotein Ib, glycoprotein IIb/IIIa, collagen and heparin. Mutations in this gene or deficiencies in this protein result in Von Willebrand's disease. VWD is characterized by frequent bleeding (gingival, minor skin quantitative lacerations, menorrhagia, etc.).
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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IgG fraction
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Storage buffer
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0.01M Na phosphate, 0.25M NaCl, pH7.6, 0.05% Na azide, 0.01% Thimerisol, 15mg/ml BSA Material safety datasheet (MSDS) for this product:
Sodium Phosphate MSDS
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Purification notes
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Whole antiserum is fractionated and then further purified by ion exchange chromatography to provide the IgG fraction of antiserum.
This fraction is essentially free of other rabbit serum proteins.
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Form
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Liquid
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Concentration
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Batch dependent within range: 7.80 - 8.60 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Von Willebrand Factor antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Von Willebrand Factor antibody images, references, products related to ab6994 and other tools.
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Von Willebrand Factor antibody images:
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 Immunocytochemistry/ Immunofluorescence - Von Willebrand Factor antibody (ab6994)
Confocal extended focus photograph of von Willebrand factor staining (red) and non-specific nuclear counterstain (green) in the adult mouse olfactory bulb. This picture shows a single major vessel within the glomerular layer of the olfactory bulb. For more details see the review of this antibody by Adam Puche. Adam Puche
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Von Willebrand Factor antibody (ab6994)
This picture shows Factor VII Immunohistochemical localization in BCG infected Guinea Pig Lung. The image was kindly supplied as part of the review submitted by Elizabeth Chlipala.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Von Willebrand Factor antibody (ab6994)
Ab6994 positively staining formaldehyde fixed paraffin embedded mouse tumour sections (1/1000).Mice were subcutaneously injected with 3t3 cells which over expressed HER2.
Secondary: Biotin conjugated horse anti rabbit (1/300).Detection was achieved using DAB and the sections were counterstained with Hematoxylin.
This image is courtesy of an Abreview submitted by Jiqiang Zhang on 16 September 2005. for further details relating to the reviewers protocol please refer to the Abreviews section of the data sheet.
See Abreview
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References for Von Willebrand Factor antibody (ab6994)
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This product has been used in: (two most recent references)
Krikun G et al. The immunoconjugate "icon" targets aberrantly expressed endothelial tissue factor causing regression of endometriosis. Am J Pathol 176:1050-6 (2010). IHC-P; Human. PubMed: 20042667
Lim JC et al. Decreased expression of multidrug efflux transporters in the brains of GSK-3beta transgenic mice. Brain Res 1276:1-10 (2009). IHC-Fr; Mouse. PubMed: 19393634
See all 29 publication references for this product.
If you publish research using ab6994 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Von Willebrand Factor
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Von Willebrand Factor antibody - more information
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Von Willebrand Factor antibody - related products:
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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