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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> tRNA synthetase
Overview
Product name
Anti-WARS2 antibody
See all WARS2 products (2) ...
Description
Mouse polyclonal to WARS2
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Full length WARS2 protein (isoform 2) (Human) (NP_957715.1)
Positive control
WARS2 transfected 293T cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 1X PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> tRNA synthetase
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Western blot - WARS2 antibody (ab70157)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab70157 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: 1/500 - 1/1000. Detects a band of approximately 23 kDa (predicted molecular weight for isoform 2: 25 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of tryptophanyl-tRNA synthetase exist, a cytoplasmic form, named WARS, and a mitochondrial form, named WARS2. This gene encodes the mitochondrial tryptophanyl-tRNA synthetase. Two alternative transcripts encoding different isoforms have been described.
Cellular localization
Mitochondrial matrix.
Alternative names
- (Mt)TrpRS antibody
- Mitochondrial tryptophanyl tRNA synthetase 2 antibody
- TrpRS antibody
see all
Database links
- Entrez Gene: 10352 Human
- Omim: 604733 Human
- SwissProt: Q9UGM6 Human
Anti-WARS2 antibody images:
Western blot - WARS2 antibody (ab70157)
All lanes : Anti-WARS2 antibody (ab70157) at 1/500 dilution
Lane 1 : WARS2 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 25 kDa
Observed band size : 23 kDa (why is the actual band size different from the predicted?)
References for Anti-WARS2 antibody (ab70157)
ab70157 has not yet been referenced specifically in any publications.
Publishing research using ab70157? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"