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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> tRNA synthetase
Overview
Product name
Anti-WARS2 antibody
See all WARS2 products (2) ...
Description
Rabbit polyclonal to WARS2
Tested applications
IHC-P, ELISA, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide (Human) conjugated to KLH. C terminal.
Positive control
Hela cell line lysates
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Ammonium Sulphate Precipitation
Purification notes
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> tRNA synthetase
Applications
Show applications key
Our Abpromise guarantee covers the use of ab71303 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/10 - 1/50.
ELISA
ELISA: 1/1000ELISA: 1/1000
WB: 1/50 - 1/100.Predicted molecular weight: 40 kDa.
Target
Relevance
Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of tryptophanyl-tRNA synthetase exist, a cytoplasmic form, named WARS, and a mitochondrial form, named WARS2. This gene encodes the mitochondrial tryptophanyl-tRNA synthetase. Two alternative transcripts encoding different isoforms have been described.
Cellular localization
Mitochondrial matrix.
Alternative names
- (Mt)TrpRS antibody
- Mitochondrial tryptophanyl tRNA synthetase 2 antibody
- TrpRS antibody
see all
Database links
- Entrez Gene: 10352 Human
- Omim: 604733 Human
- SwissProt: Q9UGM6 Human
Anti-WARS2 antibody images:
Western blot - WARS2 antibody (ab71303)
Anti-WARS2 antibody (ab71303) at 1/60 dilution + Hela cell line lysate at 35 µg
Predicted band size : 40 kDa
Observed band size : 36 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-WARS2 antibody(ab71303)
ab71303 at 1/50 dilution staining WARS2 in human lung carcinoma tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). A peroxidase conjugated secondary antibody was used followed by DAB staining.
References for Anti-WARS2 antibody (ab71303)
ab71303 has not yet been referenced specifically in any publications.
Publishing research using ab71303? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"