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Products:Signal Transduction >> Adapters >> Cytoplasmic
Overview
Product name
Anti-WASF2 antibody
Description
Mouse monoclonal to WASF2
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Horse, Chicken, Cow, Dog, Chimpanzee, Rhesus monkey, Opossum
Immunogen
Recombinant fragment with tag: DRLQVKVTQL DPKEEEVSLQ GINTRKAFRS STIQDQKLFD RNSLPVPVLE TYNTCDTPPP LNNLTPYRDD GKEALKFYTD PSYFFDLWKE KMLQDTKDIM , corresponding to amino acids 73-173 of Human WASF2
DRLQVKVTQL DPKEEEVSLQ GINTRKAFRS STIQDQKLFD RNSLPVPVLE TYNTCDTPPP LNNLTPYRDD GKEALKFYTD PSYFFDLWKE KMLQDTKDIM
Positive control
HeLa cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Isotype
IgG2b
Research areas
Cancer >> Signal transduction >> Other
Signal Transduction >> Adapters >> Cytoplasmic
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Western blot - WASF2 antibody (ab60722)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab60722 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at an assay dependent dilution. (The detection limit for recombinant tagged WASF2 is approximately 0.1ng/ml as a capture antibody.)
WB: Use a concentration of 1 - 5 µg/ml.Detects a band of approximately 73 kDa (predicted molecular weight: 54 kDa).
Target
Function
Downstream effector molecule involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Promotes formation of actin filaments. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex.
Tissue specificity
Expressed in all tissues with strongest expression in placenta, lung, and peripheral blood leukocytes, but not in skeletal muscle.
Sequence similarities
Belongs to the SCAR/WAVE family.
Contains 1 WH2 domain.
Domain
Binds and activates the Arp2/3 complex via the C-terminal domain. Interacts with actin via the WH2 domain.
Cellular localization
Cytoplasm > cytoskeleton. Cell projection > lamellipodium. At the interface between the lamellipodial actin meshwork and the membrane.
Target information above from: UniProt accessionQ9Y6W5
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DICTYOSTELIUM antibody
- Protein WAVE-2 antibody
- SCAR 2 antibody
see all
Database links
- Entrez Gene: 10163 Human
- Entrez Gene: 242687 Mouse
- Entrez Gene: 313024 Rat
- Omim: 605875 Human
- SwissProt: Q9Y6W5 Human
- SwissProt: Q3T9Z7 Mouse
- SwissProt: Q8BH43 Mouse
- SwissProt: Q5FWU0 Rat
see all
Anti-WASF2 antibody images:
Western blot - WASF2 antibody (ab60722)
Anti-WASF2 antibody (ab60722) at 1 µg/ml + HeLa cell lysate at 25 µg
Predicted band size : 54 kDa
Observed band size : 73 kDa (why is the actual band size different from the predicted?)
Additional bands at : 28 kDa. We are unsure as to the identity of these extra bands.
References for Anti-WASF2 antibody (ab60722)
ab60722 has not yet been referenced specifically in any publications.
Publishing research using ab60722? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"