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Western blot - WASF3 antibody (ab68031)
(enlarge)
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Western blot - WASF3 antibody (ab68031)
(enlarge)
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Product Name
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WASF3 antibody
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See all WASF3 antibodies (2)...
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Product type
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Primary antibodies
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Description
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Mouse polyclonal to WASF3
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Immunogen
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Full length protein, corresponding to amino acids 1-499 of Human WASF3
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Reacts with
(species key)
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Hu, Rat
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: 1/500 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
Detection pair
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Rat brain, transfected 293T cell lysate
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Cellular localization
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Cytoplasm, cytoskeleton.
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Research areas
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Cancer >> Signal transduction >> G protein signaling >> Small G proteins >> Other
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Regulators
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Binding Proteins
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Relevance
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Downstream effector molecules involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Whole antiserum
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Storage buffer
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Preservative: None
Constituents: Whole serum
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this WASF3 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for WASF3 antibody images, references, products related to ab68031 and other tools.
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WASF3 antibody images:
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 Western blot - WASF3 antibody (ab68031)
WASF3 antibody (ab68031) at 1/500 dilution + rat brain at 25 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 55 kDa
Observed band size : 70 kDa (why is the actual band size different from the predicted?)
Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands.
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Western blot - WASF3 antibody (ab68031)
All lanes : WASF3 antibody (ab68031) at 1/500 dilution
Lane 1 : WASF3 transfected 293T cell lysate
Lane 2 : non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 55 kDa
Observed band size : 55 kDa
Additional bands at : 75 kDa. We are unsure as to the identity of these extra bands.
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Search PubMed (MEDLINE) for references to WASF3
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WASF3 antibody - more information
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Customer reviews (feedback) regarding WASF3 antibody |
Customer FAQs regarding WASF3 antibody |
Protocols for WASF3 antibody |
Price and availability of products related to WASF3 antibody |
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WASF3 antibody - related products:
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WASF3 antibody - other tools:
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Contact Abcam with a Technical Enquiry about WASF3 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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