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Products:Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Binding Proteins
Overview
Product name
Anti-WASF3 antibody
See all WASF3 products (3) ...
Description
Mouse polyclonal to WASF3
Tested applications
Cross reactivity
Reacts with
Rat, Human
Immunogen
Full length protein, corresponding to amino acids 1-499 of Human WASF3
Positive control
Rat brain, transfected 293T cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
pH: 7.20
Constituent: PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Signal transduction >> G protein signaling >> Small G proteins >> Other
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Regulators
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Binding Proteins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab68031 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 2 Images
WB
Application notes
WB: 1/500 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
Detection pair
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Downstream effector molecules involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton.
Tissue specificity
Expressed in ovary and brain.
Sequence similarities
Belongs to the SCAR/WAVE family.
Contains 1 WH2 domain.
Domain
Binds the Arp2/3 complex through the C-terminal region and actin through verprolin homology (VPH) domain.
Cellular localization
Cytoplasm > cytoskeleton.
Target information above from: UniProt accessionQ9UPY6
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Protein WAVE-3 antibody
- Protein WAVE3 antibody
- SCAR3 antibody
see all
Database links
- Entrez Gene: 10810 Human
- Omim: 605068 Human
- SwissProt: Q9UPY6 Human
- Unigene: 635221 Human
Anti-WASF3 antibody images:
Western blot - WASF3 antibody (ab68031)
Anti-WASF3 antibody (ab68031) at 1/500 dilution + rat brain at 25 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 55 kDa
Observed band size : 70 kDa (why is the actual band size different from the predicted?)
Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands.
Western blot - WASF3 antibody (ab68031)
All lanes : Anti-WASF3 antibody (ab68031) at 1/500 dilution
Lane 1 : WASF3 transfected 293T cell lysate
Lane 2 : non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 55 kDa
Observed band size : 55 kDa
Additional bands at : 75 kDa. We are unsure as to the identity of these extra bands.
References for Anti-WASF3 antibody (ab68031)
ab68031 has not yet been referenced specifically in any publications.
Publishing research using ab68031? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"