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Products:Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Binding Proteins
Overview
Product name
Anti-WASF3 antibody
See all WASF3 products (3) ...
Description
Rabbit polyclonal to WASF3
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Cow, Dog
Immunogen
A synthetic peptide corresponding to a region of Human WASF3
Positive control
Fetal brain cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Signal transduction >> G protein signaling >> Small G proteins >> Other
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Regulators
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Binding Proteins
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Western blot - WASF3 antibody (ab74872)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab74872 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
ELISA
Application notes
ELISA: Use at an assay dependent dilution. Titre using peptide based assay: 1/312500.
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 55 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Downstream effector molecules involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton.
Tissue specificity
Expressed in ovary and brain.
Sequence similarities
Belongs to the SCAR/WAVE family.
Contains 1 WH2 domain.
Domain
Binds the Arp2/3 complex through the C-terminal region and actin through verprolin homology (VPH) domain.
Cellular localization
Cytoplasm > cytoskeleton.
Target information above from: UniProt accessionQ9UPY6
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Protein WAVE-3 antibody
- Protein WAVE3 antibody
- SCAR3 antibody
see all
Database links
- Entrez Gene: 10810 Human
- Entrez Gene: 245880 Mouse
- Omim: 605068 Human
- SwissProt: Q9UPY6 Human
- SwissProt: Q8VHI6 Mouse
- Unigene: 635221 Human
- Unigene: 472750 Mouse
Anti-WASF3 antibody images:
Western blot - WASF3 antibody (ab74872)
Anti-WASF3 antibody (ab74872) at 1 µg/ml (in 5% skim milk / PBS buffer) + Fetal brain cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 55 kDa
Observed band size : 55 kDa
Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.
Gel concentration: 12%
References for Anti-WASF3 antibody (ab74872)
ab74872 has not yet been referenced specifically in any publications.
Publishing research using ab74872? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"