Recombinant Anti-WASP/Wiskott-Aldrich syndrome protein antibody [EP2541Y] (ab75830)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2541Y] to WASP/Wiskott-Aldrich syndrome protein
- Suitable for: Flow Cyt (Intra), WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-WASP/Wiskott-Aldrich syndrome protein antibody [EP2541Y]
See all WASP/Wiskott-Aldrich syndrome protein primary antibodies -
Description
Rabbit monoclonal [EP2541Y] to WASP/Wiskott-Aldrich syndrome protein -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, ICC/IFmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: TF-1 and U-937 whole cell lysates. ICC/IF: TF-1 cells. Flow Cyt (Intra): U-937 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP2541Y -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab75830 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/30.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/10000 - 1/50000. Detects a band of approximately 60 kDa (predicted molecular weight: 53 kDa).
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ICC/IF |
1/50.
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Notes |
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Flow Cyt (Intra)
1/30. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/10000 - 1/50000. Detects a band of approximately 60 kDa (predicted molecular weight: 53 kDa). |
ICC/IF
1/50. |
Target
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Function
Effector protein for Rho-type GTPases, providing a link with the Arp2/3 complex that regulates the structure and dynamics of the actin cytoskeleton. Important for efficient actin polymerization. Possible regulator of lymphocyte and platelet function. -
Tissue specificity
Expressed predominantly in the thymus. Also found, to a much lesser extent, in the spleen. -
Involvement in disease
Defects in WAS are the cause of Wiskott-Aldrich syndrome (WAS) [MIM:301000]; also known as eczema-thrombocytopenia-immunodeficiency syndrome. WAS is an X-linked recessive immunodeficiency characterized by eczema, thrombocytopenia, recurrent infections, and bloody diarrhea. Death usually occurs before age 10.
Defects in WAS are the cause of thrombocytopenia type 1 (THC1) [MIM:313900]. Thrombocytopenia is defined by a decrease in the number of platelets in circulating blood, resulting in the potential for increased bleeding and decreased ability for clotting.
Defects in WAS are a cause of neutropenia severe congenital X-linked (XLN) [MIM:300299]. XLN is an immunodeficiency syndrome characterized by recurrent major bacterial infections, severe congenital neutropenia, and monocytopenia. -
Sequence similarities
Contains 1 CRIB domain.
Contains 1 WH1 domain.
Contains 1 WH2 domain. -
Domain
The WH1 (Wasp homology 1) domain may bind a Pro-rich ligand.
The CRIB (Cdc42/Rac-interactive-binding) region binds to the C-terminal WH2 domain in the autoinhibited state of the protein. Binding of Rho-type GTPases to the CRIB induces a conformation change and leads to activation. -
Cellular localization
Cytoplasm > cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 7454 Human
- Omim: 300392 Human
- SwissProt: P42768 Human
- Unigene: 2157 Human
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Alternative names
- Eczema thrombocytopenia antibody
- IMD2 antibody
- SCNX antibody
see all
Images
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All lanes : Anti-WASP/Wiskott-Aldrich syndrome protein antibody [EP2541Y] (ab75830) at 1/10000 dilution (Purified)
Lane 1 : TF-1 (Human Erythroleukemia erythroblast) whole cell lysate
Lane 2 : U-937 (Human histiocytic lymphoma monocyte) whole cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 53 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
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Immunocytochemistry analysis of TF-1 (Human bone marrow erythroblast) cells labeling WASP/Wiskott-Aldrich syndrome protein with Purified ab75830 at 1/50 dilution (5.88 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488,ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Intracellular Flow Cytometry analysis of U-937 (Human histiocytic lymphoma monocyte) cells labeling WASP/Wiskott-Aldrich syndrome protein with Purified ab75830 at 1/30 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab75830 has been referenced in 5 publications.
- Jiang J et al. Clinical and molecular characteristics of Wiskott-Aldrich Syndrome in five unrelated Chinese families. Scand J Immunol 95:e13115 (2022). PubMed: 34758123
- Magnani A et al. Long-term safety and efficacy of lentiviral hematopoietic stem/progenitor cell gene therapy for Wiskott-Aldrich syndrome. Nat Med 28:71-80 (2022). PubMed: 35075289
- Liu H et al. Clinical and genetic analysis of 2 rare cases of Wiskott-Aldrich syndrome from Chinese minorities: Two case reports. Medicine (Baltimore) 100:e25527 (2021). PubMed: 33879693
- Girón-Pérez DA et al. Myo1e modulates the recruitment of activated B cells to inguinal lymph nodes. J Cell Sci 133:N/A (2020). PubMed: 31964710
- Rai R et al. Targeted gene correction of human hematopoietic stem cells for the treatment of Wiskott - Aldrich Syndrome. Nat Commun 11:4034 (2020). PubMed: 32788576