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Western blot - WAVE 1 antibody (ab50356)

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Product Name
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WAVE 1 antibody
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See all WAVE 1 antibodies (7)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to WAVE 1
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Immunogen
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Synthetic peptide: AVEYSDSEDDSEFDEVDWLE conjugated to KLH, corresponding to C terminal amino acids 540-559 of Human WAVE 1 BLAST 'AVEYSDSEDDSEFDEVDWLE' with or 
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Reacts with
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Hu, Ms, Rat
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Predicted to react
(species key)
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Cow (Due to sequence homology)
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions WB: Use at a concentration of 0.2 - 0.4 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 62 kDa). Staining of the WAVE band is specifically inhibited with the immunizing peptide.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Rat and mouse brain cytosolic fractions.
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Cellular localization
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Cytoplasmic; Concentrated in Rac regulated membrane ruffling areas.
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Research areas
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Cancer >> Tumor biomarkers >> Other Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> Other Neuroscience >> Neurology process >> Neurogenesis Signal Transduction >> Metabolism >> Mitochondrial Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Assembly
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Relevance
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The Wiskott Aldrich syndrome family of proteins share similar domain structure, and are involved in transduction of signals from receptors on the cell surface to the actin cytoskeleton. They play an important part in cell motility. The presence of a number of different motifs suggests that they are regulated by a number of different stimuli, and interact with multiple proteins. Recent studies have demonstrated that these proteins, directly or indirectly, associate with the small GTPase, Cdc42, known to regulate formation of actin filaments, and the cytoskeletal organizing complex, Arp2/3.
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Database links
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The links below go to external sites and will open in a new browser window
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 15mM Sodium Azide Constituents: 0.01M PBS, pH 7.4 Material safety datasheet (MSDS) for this product: Sodium Azide MSDS
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Purification notes
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Affinity purified using the immunizing peptide immobilized on agarose.
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Form
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Liquid
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Concentration
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1.700 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this WAVE 1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for WAVE 1 antibody images, references, products related to ab50356 and other tools.
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WAVE 1 antibody images:
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Western blot - WAVE 1 antibody (ab50356)
All lanes : WAVE 1 antibody (ab50356) at 1/4000 dilution
Lane 1 : Mouse brain (cytosolic fraction S1)
Lane 2 : : Rat brain (cytosolic fraction S1)
Lane 3 : : Rat brain (cytosolic fraction S1)
with immunizing peptide at 20 µg/ml
Secondary Goat Anti-Rabbit IgG, Peroxidase conjugate developed using the ECL technique
Predicted band size : 62 kDa Observed band size : 75 kDa (why is the actual band size different from the predicted?)
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Search PubMed (MEDLINE) for references to WAVE 1
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WAVE 1 antibody - more information
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Customer reviews (feedback) regarding WAVE 1 antibody |
Customer FAQs regarding WAVE 1 antibody |
Protocols for WAVE 1 antibody |
Price and availability of products related to WAVE 1 antibody |
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WAVE 1 antibody - related products:
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WAVE 1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about WAVE 1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"