WAVE 1 antibody (ab50356)

Product Name 

WAVE 1 antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to WAVE 1

Immunogen 

Synthetic peptide: AVEYSDSEDDSEFDEVDWLE conjugated to KLH, corresponding to C terminal amino acids 540-559 of Human WAVE 1
BLAST 'AVEYSDSEDDSEFDEVDWLE' with or

Reacts with 

Hu, Ms, Rat

Predicted to react 
(species key)

Cow
(Due to sequence homology)

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
WB: Use at a concentration of 0.2 - 0.4 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 62 kDa). Staining of the WAVE band is specifically
inhibited with the immunizing peptide.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Rat and mouse brain cytosolic fractions.

Cellular localization 

Cytoplasmic; Concentrated in Rac regulated membrane ruffling areas.

Research areas 

Cancer >> Tumor biomarkers >> Other
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> Other
Neuroscience >> Neurology process >> Neurogenesis
Signal Transduction >> Metabolism >> Mitochondrial
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Assembly

Relevance 

The Wiskott Aldrich syndrome family of proteins share similar domain structure, and are involved in transduction of signals from receptors on the cell surface to the actin cytoskeleton. They play an important part in cell motility. The presence of a number of different motifs suggests that they are regulated by a number of different stimuli, and interact with multiple proteins. Recent studies have demonstrated that these proteins, directly or indirectly, associate with the small GTPase, Cdc42, known to regulate formation of actin filaments, and the cytoskeletal organizing complex, Arp2/3.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

Affinity purified using the immunizing peptide immobilized on agarose.

Form 

Liquid

Concentration 

1.700 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

See below for WAVE 1 antibody images, references, products related to ab50356 and other tools.

WAVE 1 antibody images:

Customer reviews (feedback) regarding WAVE 1 antibody

Customer FAQs regarding WAVE 1 antibody

Protocols for WAVE 1 antibody

Price and availability of products related to WAVE 1 antibody

WAVE 1 antibody - related products: