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Anti-WAVE 1 antibody (ab50356)

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Overview

Product name

Anti-WAVE 1 antibody
See all WAVE 1 products (7) ...

Description

Rabbit polyclonal to WAVE 1

Tested applications

WBmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Predicted to work with

Cow

Immunogen

Synthetic peptide: AVEYSDSEDDSEFDEVDWLE conjugated to KLH, corresponding to C terminal amino acids 540-559 of Human WAVE 1

AVEYSDSEDD SEFDEVDWLE

Positive control

Rat and mouse brain cytosolic fractions.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

Affinity purified using the immunizing peptide immobilized on agarose.

Clonality

Polyclonal

Isotype

IgG

  • Western blot - WAVE 1 antibody (ab50356)Western blot - WAVE 1 antibody (ab50356) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab50356 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

WB: Use at a concentration of 0.2 - 0.4 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 62 kDa). Staining of the WAVE band is specifically
inhibited with the immunizing peptide.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Downstream effector molecule involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Promotes formation of actin filaments. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex.

Tissue specificity

Highly expressed in brain. Lowly expressed in testis, ovary, colon, kidney, pancreas, thymus, small intestine and peripheral blood.

Sequence similarities

Belongs to the SCAR/WAVE family.
Contains 1 WH2 domain.

Domain

Binds the Arp2/3 complex through the C-terminal region and actin through verprolin homology (VPH) domain.

Cellular localization

Cytoplasm > cytoskeleton. Dot-like pattern in the cytoplasm. Concentrated in Rac-regulated membrane-ruffling areas.

Target information above from: UniProt accessionQ92558 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • FLJ31482 antibody
  • KIAA0269 antibody
  • Protein WAVE-1 antibody
  • Protein WAVE1 antibody
  • SCAR1 antibody
  • Similar to a plant extensin like protein antibody
  • Verprolin homology domain containing protein 1 antibody
  • Verprolin homology domain-containing protein 1 antibody
  • WAS protein family, member 1 antibody
  • Wasf1 antibody
  • WASF1_HUMAN antibody
  • WASL antibody
  • WASP family 1 antibody
  • WASP family protein member 1 antibody
  • WAVE antibody
  • WAVE1 antibody
  • Wiskott Aldrich syndrome protein family member 1 antibody
  • Wiskott-Aldrich syndrome protein family member 1 antibody
see all

Anti-WAVE 1 antibody images:

  Western blot - WAVE 1 antibody (ab50356)

Western blot - WAVE 1 antibody (ab50356)

All lanes : Anti-WAVE 1 antibody (ab50356) at 1/4000 dilution

Lane 1 : Mouse brain (cytosolic fraction S1)
Lane 2 : : Rat brain (cytosolic fraction S1)
Lane 3 : : Rat brain (cytosolic fraction S1) with immunizing peptide at 20 µg/ml

Secondary
Goat Anti-Rabbit IgG, Peroxidase conjugate
developed using the ECL technique

Predicted band size : 62 kDa
Observed band size : 75 kDa (why is the actual band size different from the predicted?)

References for Anti-WAVE 1 antibody (ab50356)

ab50356 has not yet been referenced specifically in any publications.

Publishing research using ab50356? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"