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Products:Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Assembly
Overview
Product name
Anti-WAVE 1 antibody
See all WAVE 1 products (7) ...
Description
Rabbit polyclonal to WAVE 1
Tested applications
Cross reactivity
Reacts with
Mouse, Rat, Human
Predicted to work with
Cow
Immunogen
Synthetic peptide: AVEYSDSEDDSEFDEVDWLE conjugated to KLH, corresponding to C terminal amino acids 540-559 of Human WAVE 1
AVEYSDSEDD SEFDEVDWLE
Positive control
Rat and mouse brain cytosolic fractions.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Affinity purified using the immunizing peptide immobilized on agarose.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Tumor biomarkers >> Other
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> Other
Neuroscience >> Neurology process >> Neurogenesis
Signal Transduction >> Metabolism >> Mitochondrial
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Assembly
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Western blot - WAVE 1 antibody (ab50356)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab50356 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: Use at a concentration of 0.2 - 0.4 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 62 kDa). Staining of the WAVE band is specifically
inhibited with the immunizing peptide.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Downstream effector molecule involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Promotes formation of actin filaments. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex.
Tissue specificity
Highly expressed in brain. Lowly expressed in testis, ovary, colon, kidney, pancreas, thymus, small intestine and peripheral blood.
Sequence similarities
Belongs to the SCAR/WAVE family.
Contains 1 WH2 domain.
Domain
Binds the Arp2/3 complex through the C-terminal region and actin through verprolin homology (VPH) domain.
Cellular localization
Cytoplasm > cytoskeleton. Dot-like pattern in the cytoplasm. Concentrated in Rac-regulated membrane-ruffling areas.
Target information above from: UniProt accessionQ92558
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- FLJ31482 antibody
- KIAA0269 antibody
- Protein WAVE-1 antibody
see all
Database links
- Entrez Gene: 531488 Cow
- Entrez Gene: 8936 Human
- Entrez Gene: 83767 Mouse
- Entrez Gene: 294568 Rat
- Omim: 605035 Human
- SwissProt: Q0IIJ3 Cow
- SwissProt: Q92558 Human
- SwissProt: Q8R5H6 Mouse
see all
Anti-WAVE 1 antibody images:
Western blot - WAVE 1 antibody (ab50356)
All lanes : Anti-WAVE 1 antibody (ab50356) at 1/4000 dilution
Lane 1 : Mouse brain (cytosolic fraction S1)
Lane 2 : : Rat brain (cytosolic fraction S1)
Lane 3 : : Rat brain (cytosolic fraction S1)
with immunizing peptide at 20 µg/ml
Secondary
Goat Anti-Rabbit IgG, Peroxidase conjugate
developed using the ECL technique
Predicted band size : 62 kDa
Observed band size : 75 kDa (why is the actual band size different from the predicted?)
References for Anti-WAVE 1 antibody (ab50356)
ab50356 has not yet been referenced specifically in any publications.
Publishing research using ab50356? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"