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Products:Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Assembly
Overview
Product name
Anti-WAVE 1 (phospho Y125) antibody
See all WAVE 1 products (7) ...
Description
Rabbit polyclonal to WAVE 1 (phospho Y125)
Specificity
Detects endogenous levels of WAVE1 only when phosphorylated at tyrosine 125.
Tested applications
ELISA, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic phosphopeptide derived from human WAVE1 around the phosphorylation site of tyrosine 125 (E-T-YP-D-V).
Positive control
Human colon carcinoma.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Tumor biomarkers >> Other
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> Other
Neuroscience >> Neurology process >> Neurogenesis
Signal Transduction >> Metabolism >> Mitochondrial
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Assembly
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Immunohistochemistry (Paraffin-embedded sections) - WAVE 1 (phospho Y125) antibody (ab59280)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab59280 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/5000ELISA: 1/5000
IHC-P: 1/50 - 1/100.
Target
Function
Downstream effector molecule involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Promotes formation of actin filaments. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex.
Tissue specificity
Highly expressed in brain. Lowly expressed in testis, ovary, colon, kidney, pancreas, thymus, small intestine and peripheral blood.
Sequence similarities
Belongs to the SCAR/WAVE family.
Contains 1 WH2 domain.
Domain
Binds the Arp2/3 complex through the C-terminal region and actin through verprolin homology (VPH) domain.
Cellular localization
Cytoplasm > cytoskeleton. Dot-like pattern in the cytoplasm. Concentrated in Rac-regulated membrane-ruffling areas.
Target information above from: UniProt accessionQ92558
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- FLJ31482 antibody
- KIAA0269 antibody
- Protein WAVE-1 antibody
see all
Database links
- Entrez Gene: 8936 Human
- Entrez Gene: 83767 Mouse
- Omim: 605035 Human
- SwissProt: Q92558 Human
- SwissProt: Q8R5H6 Mouse
- Unigene: 75850 Human
- Unigene: 41353 Mouse
Anti-WAVE 1 (phospho Y125) antibody images:
Immunohistochemistry (Paraffin-embedded sections) - WAVE 1 (phospho Y125) antibody (ab59280)
Ab59280 at 1/50 dilution staining human colon carcinoma without (left) and with (right) immunizing peptide; paraffin embedded.
References for Anti-WAVE 1 (phospho Y125) antibody (ab59280)
ab59280 has not yet been referenced specifically in any publications.
Publishing research using ab59280? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"