You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Related products

Reassurance, Refunds & Replacements

If your product does not perform as described on this datasheet, we will refund or replace your product...

Read our guarantee »
 

Anti-WAVE 1 (phospho Y125) antibody (ab59280)

CodeSizePriceAbpointsAvailability
    
 
  • -
  •   
  •   
  •   
  •  
  •   
  •   
Updating...

Overview

Product name

Anti-WAVE 1 (phospho Y125) antibody
See all WAVE 1 products (7) ...

Description

Rabbit polyclonal to WAVE 1 (phospho Y125)

Specificity

Detects endogenous levels of WAVE1 only when phosphorylated at tyrosine 125.

Tested applications

ELISA, IHC-Pmore details

Cross reactivity

Reacts with

Mouse, Human

Immunogen

Synthetic phosphopeptide derived from human WAVE1 around the phosphorylation site of tyrosine 125 (E-T-YP-D-V).

Positive control

Human colon carcinoma.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

  • Immunohistochemistry (Paraffin-embedded sections) - WAVE 1 (phospho Y125) antibody (ab59280)Immunohistochemistry (Paraffin-embedded sections) - WAVE 1 (phospho Y125) antibody (ab59280) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab59280 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ELISA

     ELISA: 1/5000

    ELISA: 1/5000

  • 1 Image

    IHC-P

     IHC-P: 1/50 - 1/100.

    IHC-P: 1/50 - 1/100.

Target

Function

Downstream effector molecule involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Promotes formation of actin filaments. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex.

Tissue specificity

Highly expressed in brain. Lowly expressed in testis, ovary, colon, kidney, pancreas, thymus, small intestine and peripheral blood.

Sequence similarities

Belongs to the SCAR/WAVE family.
Contains 1 WH2 domain.

Domain

Binds the Arp2/3 complex through the C-terminal region and actin through verprolin homology (VPH) domain.

Cellular localization

Cytoplasm > cytoskeleton. Dot-like pattern in the cytoplasm. Concentrated in Rac-regulated membrane-ruffling areas.

Target information above from: UniProt accessionQ92558 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • FLJ31482 antibody
  • KIAA0269 antibody
  • Protein WAVE-1 antibody
  • Protein WAVE1 antibody
  • SCAR1 antibody
  • Similar to a plant extensin like protein antibody
  • Verprolin homology domain containing protein 1 antibody
  • Verprolin homology domain-containing protein 1 antibody
  • WAS protein family, member 1 antibody
  • WASF1 antibody
  • WASF1_HUMAN antibody
  • WASL antibody
  • WASP family 1 antibody
  • WASP family protein member 1 antibody
  • WAVE antibody
  • WAVE1 antibody
  • Wiskott Aldrich syndrome protein family member 1 antibody
  • Wiskott-Aldrich syndrome protein family member 1 antibody
see all

Anti-WAVE 1 (phospho Y125) antibody images:

  Immunohistochemistry (Paraffin-embedded sections) - WAVE 1 (phospho Y125) antibody (ab59280)

Immunohistochemistry (Paraffin-embedded sections) - WAVE 1 (phospho Y125) antibody (ab59280)

Ab59280 at 1/50 dilution staining human colon carcinoma without (left) and with (right) immunizing peptide; paraffin embedded.

References for Anti-WAVE 1 (phospho Y125) antibody (ab59280)

ab59280 has not yet been referenced specifically in any publications.

Publishing research using ab59280? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"