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Immunohistochemistry (Paraffin-embedded sections) - WAVE 1 (phospho Y125) antibody (ab59280)
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Product Name
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WAVE 1 (phospho Y125) antibody
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See all WAVE 1 antibodies (7)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to WAVE 1 (phospho Y125)
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Immunogen
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Synthetic phosphopeptide derived from human WAVE1 around the phosphorylation site of tyrosine 125 (E-T-YP-D-V).
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Reacts with
(species key)
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Hu, Ms
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Specificity
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Detects endogenous levels of WAVE1 only when phosphorylated at tyrosine 125.
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Tested applications
(see key)
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ELISA, IHC-P
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/5000.
IHC-P: 1/50 - 1/100.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human colon carcinoma.
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Cellular localization
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Cytoplasmic; Concentrated in Rac regulated membrane ruffling areas.
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Research areas
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Cancer >> Tumor biomarkers >> Other
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> Other
Neuroscience >> Neurology process >> Neurogenesis
Signal Transduction >> Metabolism >> Mitochondrial
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Assembly
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Relevance
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The Wiskott Aldrich syndrome family of proteins share similar domain structure, and are involved in transduction of signals from receptors on the cell surface to the actin cytoskeleton. They play an important part in cell motility. The presence of a number of different motifs suggests that they are regulated by a number of different stimuli, and interact with multiple proteins. Recent studies have demonstrated that these proteins, directly or indirectly, associate with the small GTPase, Cdc42, known to regulate formation of actin filaments, and the cytoskeletal organizing complex, Arp2/3.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this WAVE 1 (phospho Y125) antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for WAVE 1 (phospho Y125) antibody images, references, products related to ab59280 and other tools.
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WAVE 1 (phospho Y125) antibody images:
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 Immunohistochemistry (Paraffin-embedded sections) - WAVE 1 (phospho Y125) antibody (ab59280)
Ab59280 at 1/50 dilution staining human colon carcinoma without (left) and with (right) immunizing peptide; paraffin embedded.
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Search PubMed (MEDLINE) for references to WAVE 1
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WAVE 1 (phospho Y125) antibody - more information
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Customer reviews (feedback) regarding WAVE 1 (phospho Y125) antibody |
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WAVE 1 (phospho Y125) antibody - related products:
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WAVE 1 (phospho Y125) antibody - other tools:
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