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Read our guarantee »Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Splicing
Anti-WBP11 antibody
See all WBP11 products (2) ...
Mouse polyclonal to WBP11
Reacts with
Human
Full length human WBP11 protein (NP_057396)
WBP11 transfected 293T cell lysate.
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: None
Constituents: 1X PBS, pH 7.2
Concentration information loading...
Protein G purified
Polyclonal
IgG
Western blot - WBP11 antibody (ab69352)
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Our Abpromise guarantee covers the use of ab69352 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 100 kDa (predicted molecular weight: 70 kDa).
WBP11 (WW domain-binding protein 11) colocalizes with mRNA splicing factors and intermediate filament-containing perinuclear networks. It may play a role in the regulation of pre-mRNA processing.
Nuclear and Cytoplasmic. Note: Predominantly located in the nucleus with granular heterogeneous distribution. Excluded from nucleoli in interphase cells, distributed throughout cytoplasm in dividing cells. Co-localized with SC35 and U2B in the nucleus. In the cytoplasm, associates with the intermediate filament protein vimentin.
Western blot - WBP11 antibody (ab69352)

All lanes : Anti-WBP11 antibody (ab69352) at 1/500 dilution
Lane 1 : WBP11 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 70 kDa
Observed band size : 100 kDa (why is the actual band size different from the predicted?)
ab69352 has not yet been referenced specifically in any publications.
Publishing research using ab69352? Please let us know so that we can cite the reference in this datasheet
Concentration of lot no. is
Concentration not available for this lot.
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All lanes : Anti-WBP11 antibody (ab69352) at 1/500 dilution
Lane 1 : WBP11 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 70 kDa
Observed band size : 100 kDa (why is the actual band size different from the predicted?)
0
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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