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Read our guarantee »Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Splicing
Anti-WBP11 antibody
See all WBP11 products (2) ...
Rabbit polyclonal to WBP11
Reacts with
Human
Predicted to work with
Mouse, Rat, Chicken, Dog, Fruit fly (Drosophila melanogaster), Zebrafish
Synthetic peptide corresponding to a region within N terminal amino acids 2-51 (GRRSTSSTKS GKFMNPTDQA RKEARKRELK KNKKQRMMVR AAVLKMKDPK) of Human WBP11 (NP_057396).
GRRSTSSTKS GKFMNPTDQA RKEARKRELK KNKKQRMMVR AAVLKMKDPK
HeLa cell lysate.
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration information loading...
Immunogen affinity purified
Polyclonal
IgG
Western blot - WBP11 antibody (ab85563)
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Our Abpromise guarantee covers the use of ab85563 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlPredicted molecular weight: 70 kDa.
WBP11 (WW domain-binding protein 11) colocalizes with mRNA splicing factors and intermediate filament-containing perinuclear networks. It may play a role in the regulation of pre-mRNA processing.
Nuclear and Cytoplasmic. Note: Predominantly located in the nucleus with granular heterogeneous distribution. Excluded from nucleoli in interphase cells, distributed throughout cytoplasm in dividing cells. Co-localized with SC35 and U2B in the nucleus. In the cytoplasm, associates with the intermediate filament protein vimentin.
Western blot - WBP11 antibody (ab85563)

Anti-WBP11 antibody (ab85563) at 1 µg/ml (in 5% skim milk / PBS buffer) + HeLa cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 70 kDa
Observed band size : 75 kDa (why is the actual band size different from the predicted?)
Gel concentration: 12%
ab85563 has not yet been referenced specifically in any publications.
Publishing research using ab85563? Please let us know so that we can cite the reference in this datasheet
Concentration of lot no. is
Concentration not available for this lot.
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Anti-WBP11 antibody (ab85563) at 1 µg/ml (in 5% skim milk / PBS buffer) + HeLa cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 70 kDa
Observed band size : 75 kDa (why is the actual band size different from the predicted?)
Gel concentration: 12%
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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