|
|
|
|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - WDFY3 antibody (ab84888)
(enlarge)
|
|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - WDFY3 antibody (ab84888)
(enlarge)
|
|
|
|
|
Product Name
|
WDFY3 antibody
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rabbit polyclonal to WDFY3
|
|
Immunogen
|
Synthetic peptide corresponding to a region between residue 500 and 550 of human WDFY3 (accession number: NP_055806.2)
|
|
Reacts with
(species key)
|
Hu
|
|
Tested applications
(see key)
|
IHC-P
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
IHC-P: 1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Positive control
(see definition)
|
Human Controls: Breast Carcinoma, Ovarian Carcinoma, Placenta, Prostate Carcinoma
|
|
Cellular localization
|
Membrane; Peripheral membrane protein; Cytoplasmic side. Note: Colocalizes with autophagic structures in starved cells.
|
|
Research areas
|
Cardiovascular >> Heart >> Autophagy >> Other
|
|
Relevance
|
WDFY3 ( WD repeat and FYVE domain containing protein 3) was identified in a screen for phosphatidylinositol 3 phosphate [PtdIns(3)P] binding proteins. The FYVE domain is a zinc binding domain found in many PtdIns(3) binding proteins and is proposed to target proteins to membrane lipids. WDFY3 has been shown localized to the nuclear membrane and within autophagic membranes and it is proposed to function as a link between protein aggregates and the autophagic machinery. WDFY3 is also known as autophagy linked FYVE protein (Alfy).
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window Entrez Gene | | 23001 (Human)
|
| |
|
|
Raised in
|
Rabbit
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
Immunogen affinity purified
|
|
Storage buffer
|
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
|
|
Purification notes
|
Purified using an epitope specific to WDFY3 immobilized on solid support
|
|
Form
|
Liquid
|
|
Storage instructions
|
Store at +4°C.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this WDFY3 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for WDFY3 antibody images, references, products related to ab84888 and other tools.
|
| |
WDFY3 antibody images:
|
 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - WDFY3 antibody (ab84888)
Detection of Human WDFY3 in a formalin-fixed paraffin-embedded section of human ovarian carcinomaby by Immunohistochemistry using ab84888 at a dilution of 1:250. Detection: DAB staining.
|
| |
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - WDFY3 antibody (ab84888)
Detection of Human WDFY3 in a formalin-fixed paraffin-embedded section of human breast carcinomaby by Immunohistochemistry using ab84888 at a dilution of 1:250. Detection: DAB staining.
|
| |
| |
|
Search PubMed (MEDLINE) for references to WDFY3
|
| |
WDFY3 antibody - more information
|
Customer reviews (feedback) regarding WDFY3 antibody |
Customer FAQs regarding WDFY3 antibody |
Protocols for WDFY3 antibody |
Price and availability of products related to WDFY3 antibody |
| |
WDFY3 antibody - related products:
|
|
|
| |
WDFY3 antibody - other tools:
|
Contact Abcam with a Technical Enquiry about WDFY3 antibody |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
