Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Cardiovascular >> Heart >> Autophagy >> Other
Overview
Product name
Anti-WDFY3 antibody
Description
Rabbit polyclonal to WDFY3
Tested applications
IHC-P, ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide corresponding to a region between residue 500 and 550 of human WDFY3 (accession number: NP_055806.2)
Positive control
Human Controls: Breast Carcinoma, Ovarian Carcinoma, Placenta, Prostate Carcinoma
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified using an epitope specific to WDFY3 immobilized on solid support
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Heart >> Autophagy >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab84888 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/100 - 1/500.(Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.)ICC/IF: Use at an assay dependent dilution.
Target
Relevance
WDFY3 ( WD repeat and FYVE domain containing protein 3) was identified in a screen for phosphatidylinositol 3 phosphate [PtdIns(3)P] binding proteins. The FYVE domain is a zinc binding domain found in many PtdIns(3) binding proteins and is proposed to target proteins to membrane lipids. WDFY3 has been shown localized to the nuclear membrane and within autophagic membranes and it is proposed to function as a link between protein aggregates and the autophagic machinery. WDFY3 is also known as autophagy linked FYVE protein (Alfy).
Cellular localization
Membrane; Peripheral membrane protein; Cytoplasmic side. Note: Colocalizes with autophagic structures in starved cells.
Alternative names
- Alfy antibody
- Autophagy linked FYVE protein antibody
- KIAA0993 antibody
see all
Database links
- Entrez Gene: 23001 Human
- SwissProt: Q8IZQ1 Human
Anti-WDFY3 antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - WDFY3 antibody (ab84888)
Detection of Human WDFY3 in a formalin-fixed paraffin-embedded section of human ovarian carcinomaby by Immunohistochemistry using ab84888 at a dilution of 1:250. Detection: DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - WDFY3 antibody (ab84888)
Detection of Human WDFY3 in a formalin-fixed paraffin-embedded section of human breast carcinomaby by Immunohistochemistry using ab84888 at a dilution of 1:250. Detection: DAB staining.
Immunocytochemistry/ Immunofluorescence - WDFY3 antibody (ab84888)
ab84888 staining WDFY3 in Human HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with Triton X-100 0.5% in PBS and blocked with 2% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/50 in PBS + 2% BSA) for 1 hour at 25°C. An Alexa Fluor®594-conjugated Donkey anti-rabbit monoclonal (1/2000) was used as the secondary antibody.
This image is courtesy of an anonymous Abreview
References for Anti-WDFY3 antibody (ab84888)
ab84888 has not yet been referenced specifically in any publications.
Publishing research using ab84888? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"