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Products:Developmental Biology >> Reproduction >> Germ cell markers
Overview
Product name
Anti-WDR33 antibody
See all WDR33 products (3) ...
Description
Rabbit polyclonal to WDR33
Tested applications
WB, IPmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide corresponding to a region between residues 1286 and 1336 of human WDR33 (NP_060853.3).
Positive control
HeLa and 293T whole cell lysates.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab72115 was affinity purified using an epitope specific to WDR33 immobilized on solid support.
Clonality
Polyclonal
Isotype
IgG
Research areas
Applications
Show applications key
Our Abpromise guarantee covers the use of ab72115 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000 - 1/10000.Detects a band of approximately 180 kDa (predicted molecular weight: 146 kDa).
IP: Use at 2-5 µg/mg of lysate.
Target
Tissue specificity
Most highly expressed in testis.
Sequence similarities
Contains 1 collagen-like domain.
Contains 7 WD repeats.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ9C0J8
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- WDC146 antibody
- WDR 33 antibody
- WD repeat containing protein 33 antibody
see all
Database links
- Entrez Gene: 55339 Human
- SwissProt: Q6NUQ0 Human
- SwissProt: Q9C0J8 Human
- Unigene: 554831 Human
Anti-WDR33 antibody images:
Western blot - WDR33 antibody (ab72115)
All lanes : Anti-WDR33 antibody (ab72115) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg/ml
Lane 2 : HeLa whole cell lysate at 15 µg/ml
Lane 3 : HeLa whole cell lysate at 5 µg/ml
Lane 4 : 293T whole cell lysate at 50 µg/ml
developed using the ECL technique
Predicted band size : 146 kDa
Observed band size : 180 kDa (why is the actual band size different from the predicted?)
Exposure time : 10 seconds
Immunoprecipitation - WRD33 antibody (ab72115)
Detection of WDR33 by Western Blot of Immunprecipitate.
ab72115 at 1µg/ml staining WDR33 in HeLa whole cell lysate immunoprecipitated using ab72115 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds.
References for Anti-WDR33 antibody (ab72115)
ab72115 has not yet been referenced specifically in any publications.
Publishing research using ab72115? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"