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Western blot - WDR34 antibody (ab81030)
Western blot - WDR34 antibody (ab81030)
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Product Name 

WDR34 antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to WDR34

Immunogen 

Synthetic peptide corresponding to a region within C terminal amino acids 431-480 (QLSLKYLFAV RWSPVRPLVF AAASGKGDVQ LFDLQKSSQK PTVLIKQTQD) of human WDR34 (NP_443076).
BLAST 'QLSLKYLFAV RWSPVRPLVF AAASGKGDVQ LFDLQKSSQK PTVLIKQTQD ' with BLAST the sequence with ExPASy or BLAST the sequence with NCBI

Reacts with 

Hu, Ms, Rat

Predicted to react 
(species key)

Cow
(Due to sequence homology)

Tested applications 
(see key)

ELISA, WB


Abreviews 

Customer reviews
(see key)


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Application notes 
(see key)

Recommended dilutions
ELISA: Use at an assay dependent dilution. Titre using peptide based assay: 1/62500.
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 58 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Fetal Brain lysate.

Research areas 

Immunology >> Innate Immunity >> TLR Signaling
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> NFkB Pathway
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> MAPK Pathway

Relevance 

WDR34 is a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation.

 

WDR34 antibody (ab81030)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

89891    (Human)
71820    (Mouse)
296618    (Rat)

SwissProt

    

Q96EX3    (Human)
Q5U4F6    (Mouse)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: None
Constituents: 2% Sucrose, PBS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this WDR34 antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for WDR34 antibody images, references, products related to ab81030 and other tools.

 

WDR34 antibody images:

Product image  Western blot - WDR34 antibody (ab81030)

Western blot - WDR34 antibody (ab81030)

WDR34 antibody (ab81030) at 1 µg/ml (in 5% skim milk / PBS buffer) + Fetal Brain lysate at 10 µg

Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution

Predicted band size : 58 kDa
Observed band size : 58 kDa
Additional bands at : 90 kDa. We are unsure as to the identity of these extra bands.

Gel concentration: 12%

 
 

Search PubMed (MEDLINE) for references to WDR34

 

WDR34 antibody - more information

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WDR34 antibody - related products:

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