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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> tRNAs
Overview
Product name
Anti-WDR4 antibody
See all WDR4 products (6) ...
Description
Rabbit polyclonal to WDR4
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Cow, Dog
Immunogen
A synthetic peptide corresponding to a region within the N-terminal amino acids 35-84 (FIYDCSAAEK KSQENKGEDA PLDQGSGAIL ASTFSKSGSY FALTDDSKRL) of Human WDR4 (NP_061139).
FIYDCSAAEK KSQENKGEDA PLDQGSGAIL ASTFSKSGSY FALTDDSKRL
Positive control
Human Placenta lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> tRNAs
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Western blot - WDR4 antibody (ab83552)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab83552 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
ELISA
Application notes
ELISA: Use at an assay dependent dilution. ELISA titre using peptide based assay 1/62500.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 45 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Required for the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA. In the complex, it is required to stabilize and induce conformational change of the catalytic subunit.
Pathway
tRNA modification; N(7)-methylguanine-tRNA biosynthesis.
Sequence similarities
Belongs to the WD repeat TRM82 family.
Contains 4 WD repeats.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP57081
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- OTTHUMP00000109401 antibody
- OTTHUMP00000109402 antibody
- TRM82 antibody
see all
Database links
- Entrez Gene: 10785 Human
- Entrez Gene: 57773 Mouse
- Entrez Gene: 690032 Rat
- Omim: 605924 Human
- SwissProt: P57081 Human
- SwissProt: Q9BVM5 Human
- SwissProt: Q80V01 Mouse
- SwissProt: Q9EP82 Mouse
see all
Anti-WDR4 antibody images:
Western blot - WDR4 antibody (ab83552)
Anti-WDR4 antibody (ab83552) at 1 µg/ml + placenta lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 45 kDa
Observed band size : 50 kDa (why is the actual band size different from the predicted?)
Gel concentration 12%
References for Anti-WDR4 antibody (ab83552)
ab83552 has not yet been referenced specifically in any publications.
Publishing research using ab83552? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"