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Western blot - WDR4 antibody (ab83552)
(enlarge)
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Product Name
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WDR4 antibody
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See all WDR4 antibodies (4)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to WDR4
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Immunogen
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A synthetic peptide corresponding to a region within the N-terminal amino acids 35-84 (FIYDCSAAEK KSQENKGEDA PLDQGSGAIL ASTFSKSGSY FALTDDSKRL) of Human WDR4 (NP_061139).
BLAST 'FIYDCSAAEK KSQENKGEDA PLDQGSGAIL ASTFSKSGSY FALTDDSKRL ' with  or 
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at an assay dependent dilution. ELISA titre using peptide based assay 1/62500.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 45 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human Placenta lysate.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> DNA / RNA >> Translation >> tRNAs
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Relevance
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WDR4 is a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. WDR4 is required for 7-methylguanosine modification of tRNA. This protein is excluded as a candidate for a form of nonsyndromic deafness (DFNB10), but is still a candidate for other disorders mapped to 21q22.3 as well as for the development of Down syndrome phenotypes. Alternative splicing of this gene generates at least 3 transcript variants, two of which diverge at the 3' UTR and thus encode the same protein products.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: None
Constituents: 2% Sucrose, PBS
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Form
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Lyophilised
Add 50 µl of distilled water. Final antibody concentration is 1 mg/ml.
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this WDR4 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for WDR4 antibody images, references, products related to ab83552 and other tools.
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WDR4 antibody images:
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 Western blot - WDR4 antibody (ab83552)
WDR4 antibody (ab83552) at 1 µg/ml + placenta lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 45 kDa
Observed band size : 50 kDa (why is the actual band size different from the predicted?)
Gel concentration 12%
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Search PubMed (MEDLINE) for references to WDR4
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WDR4 antibody - more information
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Customer reviews (feedback) regarding WDR4 antibody |
Customer FAQs regarding WDR4 antibody |
Protocols for WDR4 antibody |
Price and availability of products related to WDR4 antibody |
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WDR4 antibody - related products:
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WDR4 antibody - other tools:
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Contact Abcam with a Technical Enquiry about WDR4 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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