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Overview

  • Product nameWDR45L protein (Tagged-His Tag)
  • Protein descriptionRecombinant fragment, corresponding to amino acids 139-344 of Human WDR45L with N terminal His tag; 206 amino acids, 25kDa (22.4kDa predicted from sequence). UniProt ID = Q5MNZ6.
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 104 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Additional notesProtein Identity confirmed by Mass Spectrometry (MS/MS) (acquired on initial reference batch).
  • Sequence notesYNPKGLCVLCPNSNNSLLAFPGTHTGHVQLVDLASTEKPP VDIPAHEGVLSCIALNLQGT RIATASEKGTLIRIFDTS SGHLIQELRRGSQAANIYCINFNQDASLICVSSDHGTVHI FA AEDPKRNKQSSLASASFLPKYFSSKWSFSKFQVPSG SPCICAFGTEPNAVIAICADGSYY KFLFNPKGECIRDV YAQFLEMTDDKL
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab92189 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
    MS
  • Application notesMS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        WD repeat domain phosphoinositide-interacting protein 3WD repeat protein 45 likeWD repeat-containing protein 45-like
        WDR4 likeWDR45 like proteinWDR45-like proteinwdr45lWIPI 3WIPI-3WIPI3WIPI3_HUMANWIPI49 like proteinWIPI49-like protein
      see all
  • Tissue specificityUbiquitously expressed. Highly expressed in heart, skeletal muscle and pancreas. Up-regulated in a variety of tumor tissues including ovarian and uterine cancers.
  • Sequence similaritiesBelongs to the WD repeat SVP1 family.
    Contains 2 WD repeats.

    • Target information above from: UniProt accession Q5MNZ6 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    WDR45L protein (Tagged-His Tag) images

    • SDS-PAGE - WDR45L protein (Tagged-His Tag) (ab92189)
      SDS-PAGE - WDR45L protein (Tagged-His Tag) (ab92189)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for WDR45L protein (Tagged-His Tag) (ab92189)

    ab92189 has not yet been referenced specifically in any publications.

    Publishing research using ab92189 ? Please let us know so that we can cite the reference in this datasheet

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