Anti-WDR5 antibody [2C2] (ab56919)
Key features and details
- Mouse monoclonal [2C2] to WDR5
- Suitable for: WB, ICC/IF, ChIP, Flow Cyt
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-WDR5 antibody [2C2]
See all WDR5 primary antibodies -
Description
Mouse monoclonal [2C2] to WDR5 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IF, ChIP, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment, corresponding to amino acids 16-121 of Human WDR5
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General notes
This product was changed from ascites to tissue culture supernatant on 24/1/19. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4 -
Concentration information loading...
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Purity
Tissue culture supernatant -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
2C2 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab56919 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
Use at an assay dependent concentration. Predicted molecular weight: 36 kDa.
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ICC/IF |
Use at an assay dependent concentration.
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ChIP |
Use at an assay dependent concentration. PubMed: 20864036
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Flow Cyt |
Use at an assay dependent concentration.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 36 kDa. |
ICC/IF
Use at an assay dependent concentration. |
ChIP
Use at an assay dependent concentration. PubMed: 20864036 |
Flow Cyt
Use at an assay dependent concentration. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
Target
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Function
Contributes to histone modification. May position the N-terminus of histone H3 for efficient trimethylation at 'Lys-4'. As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues. May regulate osteoblasts differentiation. -
Sequence similarities
Belongs to the WD repeat WDR5/wds family.
Contains 7 WD repeats. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 11091 Human
- Omim: 609012 Human
- SwissProt: P61964 Human
- Unigene: 397638 Human
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Alternative names
- 2410008O07Rik antibody
- AA408785 antibody
- AA960360 antibody
see all
Images
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WDR5 antibody (ab56919) at 1μg/lane + Hs 181 S cell lysate at 25μg/lane.
This image was generated using the ascites version of the product.
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Overlay histogram showing HeLA cells stained with ab56919 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56919, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.
This image was generated using the ascites version of the product.
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HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for WDR5 (green) using ab56919 at 10 µg/ml in ICC/IF.
This image was generated using the ascites version of the product.
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Anti-WDR5 antibody [2C2] (ab56919) at 1 µg/ml + Hs 181.Tes cell lysate
Predicted band size: 36 kDaThis image was generated using the ascites version of the product.
Datasheets and documents
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Datasheet download
References (33)
ab56919 has been referenced in 33 publications.
- Zhou T et al. Lupus enhancer risk variant causes dysregulation of IRF8 through cooperative lncRNA and DNA methylation machinery. Nat Commun 13:1855 (2022). PubMed: 35388006
- Zhang C et al. Histone methyltransferase MLL1 drives renal tubular cell apoptosis by p53-dependent repression of E-cadherin during cisplatin-induced acute kidney injury. Cell Death Dis 13:770 (2022). PubMed: 36068197
- Yang B et al. Localization of the WD repeat-containing protein 5 to the Virion Assembly Compartment Facilitates Human Cytomegalovirus Assembly. J Virol 95:N/A (2021). PubMed: 33504601
- Wong CH et al. Ectopic HOTTIP expression induces noncanonical transactivation pathways to promote growth and invasiveness in pancreatic ductal adenocarcinoma. Cancer Lett 477:1-9 (2020). PubMed: 32120024
- Luo H et al. HOTTIP lncRNA Promotes Hematopoietic Stem Cell Self-Renewal Leading to AML-like Disease in Mice. Cancer Cell 36:645-659.e8 (2019). PubMed: 31786140