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Overview
Product name
Anti-WDR5 antibody
See all WDR5 products (5) ...
Description
Rabbit polyclonal to WDR5
Tested applications
Cross reactivity
Reacts with
Mouse, Rat, Human
Predicted to work with
Chicken, Cow, Xenopus laevis, Zebrafish
Does not react with
Fruit fly (Drosophila melanogaster)
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Human WDR5.
(Peptide available as ab24388.)
Positive control
HeLa whole cell and nuclear extracts.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
-
Western blot - WDR5 antibody (ab22512)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab22512 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa). Can be blocked with WDR5 peptide (ab24388). ab22512 also recognises bands at 40 kDa and 50 kDa, which may be modified forms of WDR5, we are currently characterising the antibody further in house.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Contributes to histone modification. May position the N-terminus of histone H3 for efficient trimethylation at 'Lys-4'. As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. May regulate osteoblasts differentiation.
Sequence similarities
Belongs to the WD repeat WDR5/wds family.
Contains 7 WD repeats.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP61964
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 2410008O07Rik antibody
- AA408785 antibody
- AA960360 antibody
see all
Database links
- Entrez Gene: 11091 Human
- Entrez Gene: 140858 Mouse
- Entrez Gene: 362093 Rat
- Omim: 609012 Human
- SwissProt: P61964 Human
- SwissProt: P61965 Mouse
- SwissProt: Q498M4 Rat
- Unigene: 397638 Human
see all
Anti-WDR5 antibody images:
Western blot - WDR5 antibody (ab22512)
All lanes : Anti-WDR5 antibody (ab22512) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole cell lysate
Lane 3 : Brain (Rat) Whole Cell Lysate - normal tissue
Lysates/proteins at 20 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Predicted band size : 36 kDa
Observed band size : 36 kDa
Additional bands at : 30 kDa,48 kDa (possible post-translational modification),55-60 kDa. We are unsure as to the identity of these extra bands.
References for Anti-WDR5 antibody (ab22512)
This product has been referenced in:
- Benedikt Aet al. The leukemogenic AF4-MLL fusion protein causes P-TEFb kinase activation and altered epigenetic signatures. Leukemia 25:135-44 (2011). WB; Human.Read more (PubMed: 21030982) »
- Woo AJet al. Role of ZBP-89 in human globin gene regulation and erythroid differentiation. Blood 118:3684-93 (2011).Read more (PubMed: 21828133) »
See all 10 publications for this product
Publishing research using ab22512? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"