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Products:Microbiology >> Interspecies Interaction >> Host Virus Interaction
Overview
Product name
Anti-WIPF1 antibody
See all WIPF1 products (2) ...
Description
Goat polyclonal to WIPF1
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Dog
Immunogen
Peptide with sequence C-KLARNESRSGSNRRE, from the C Terminus of the protein sequence according to NP_003378.3; NP_001070737.1.
C-KLARNESR SGSNRRE
Positive control
Human Lymph Node, Spleen and Tonsil lysates
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab63944 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Assembly
Microbiology >> Interspecies Interaction >> Host Virus Interaction
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Western blot - WIPF1 antibody (ab63944)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab63944 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.03 - 0.1 µg/ml.Detects a band of approximately 55 kDa (predicted molecular weight: 51 kDa).
Target
Function
May have direct activity on the actin cytoskeleton. Induces actin polymerization and redistribution. Contributes with NCK1 and GRB2 in the recruitment and activation of WASL. May participate in regulating the subcellular localization of WASL, resulting in the disassembly of stress fibers in favor of filopodia formation (By similarity). Plays an important role in the intracellular motility of vaccinia virus by functioning as an adapter for recruiting WASL to vaccinia virus.
Tissue specificity
Highly expressed in peripheral blood mononuclear cells, spleen, placenta, small intestine, colon and thymus. Lower expression in ovary, heart, brain, lung, liver, skeletal muscle, kidney, pancreas, prostate and testis.
Sequence similarities
Belongs to the verprolin family.
Contains 1 WH2 domain.
Domain
Binds to WAS within the N-terminal region 170, at a site distinct from the CDC42-binding site.
Cellular localization
Cytoplasmic vesicle. Cytoplasm > cytoskeleton. Vesicle surfaces and along actin tails. Co-localized with actin stress fibers. When co-expressed with WASL, no longer associated with actin filaments but accumulated in perinuclear and cortical areas like WASL.
Target information above from: UniProt accessionO43516
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Protein PRPL-2 antibody
- PRPL-2 protein antibody
- WAS/WASL interacting protein family member 1 antibody
see all
Database links
- Entrez Gene: 7456 Human
- Entrez Gene: 215280 Mouse
- Entrez Gene: 117538 Rat
- Omim: 602357 Human
- SwissProt: O43516 Human
- SwissProt: Q8K1I7 Mouse
- SwissProt: Q6IN36 Rat
- Unigene: 128067 Human
see all
Anti-WIPF1 antibody images:
Western blot - WIPF1 antibody (ab63944)
Anti-WIPF1 antibody (ab63944) at 0.03 µg/ml + Human tonsil lysate in RIPA buffer at 35 µg
developed using the ECL technique
Predicted band size : 51 kDa
Observed band size : 55 kDa (why is the actual band size different from the predicted?)
Primary incubation was 1 hour.
References for Anti-WIPF1 antibody (ab63944)
ab63944 has not yet been referenced specifically in any publications.
Publishing research using ab63944? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"