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Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human WISP1.
(Peptide available as ab24414.)
Our Abpromise guarantee covers the use of ab10737 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|WB||1/500. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).Can be blocked with Human WISP1 peptide (ab24414).|
|IHC-P||1/75. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.|
Image courtesy of Human Protein Atlas
ab10737 staining WISP1 in human bone marrow, showing staining with haemapoietic tissue and not apidose tissue. Paraffin embedded human bome marrow tissue was incubated with ab10737 (1/75 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
ab10737 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org
ICC/IF image of ab10737 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab10737 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"