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Overview

  • Product nameWISP2 protein (Active)See all WISP2 proteins and peptides ...
  • Protein descriptionRecombinant Human WISP2, a 24.8 kDa protein of 228 amino acid residues.
  • Expression hostE. coli

    • Properties

  • Purity> 95 % by SDS-PAGE
  • Purification notesEndotoxin level is less than 0.1 ng per µg (1EU/µg).
  • Biological activityBiological Activity :The ED50 was determined by its ability to inhibit IGF-II induced proliferation of MCF-7 is between 10-20 ng/ml in the presence of 15 ng/ml of human IGF-II.
  • FormLyophilised
  • Storage instructionsThe lyophilized protein is stable for a few weeks at room temperature, but best stored at -20°C.This product is an active protein and may elicit a biological response in vivo, handle with caution.
  • Concentration information loading...
  • Sequence notesMQLCPTPCTC PWPPPRCPLG VPLVLDGCGC CRVCARRLGE PCDQLHVCDA SQGLVCQPGA GPGGRGALCL LAEDDSSCEV NGRLYREGET FQPHCSIRCR CEDGGFTCVP LCSEDVRLPS WDCPHPRRVE VLGKCCPEWV CGQGGGLGTQ PLPAQGPQFS GLVSSLPPGV PCPEWSTAWG PCSTTCGLGM ATRVSNQNRF CRLETQRRLC LSRPCPPSRG RSPQNSAF
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab50040 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    WB WB: Use at an assay dependent concentration. Ab50040 can be used as a WB positive control in conjunction with ab38317.
    SDS-PAGE SDS-PAGE: Use at an assay dependent dilution.
    Functional Studies FuncS: Use at an assay dependent dilution.
  • Application notesThis peptide can be used with studies using ab123375.

    • Protein info

    • Alternative names
        CCN family member 5CCN5Connective tissue growth factor like protein
        Connective tissue growth factor related protein 58Connective tissue growth factor-like proteinConnective tissue growth factor-related protein 58CT58CTGF LCTGF-LWISP 2WISP-2Wisp2WISP2_HUMANWNT1 inducible signaling pathway protein 2WNT1-inducible-signaling pathway protein 2
      see all
  • FunctionMay play an important role in modulating bone turnover. Promotes the adhesion of osteoblast cells and inhibits the binding of fibrinogen to integrin receptors. In addition, inhibits osteocalcin production.
  • Tissue specificityExpressed in primary osteoblasts, fibroblasts, ovary, testes, and heart.
  • Sequence similaritiesBelongs to the CCN family.
    Contains 1 IGFBP N-terminal domain.
    Contains 1 TSP type-1 domain.
    Contains 1 VWFC domain.
  • Cellular localizationSecreted.

    • Target information above from: UniProt accession O76076 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    WISP2 protein (Active) images

    • Western blot - Anti-WISP2 antibody (ab38317)
      Western blot - Anti-WISP2 antibody (ab38317)
      Anti-WISP2 antibody (ab38317) at 1/100 dilution + WISP2 protein (Active) (ab50040) at 0.01 µg

      Secondary
      Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
      developed using the ECL technique

      Performed under reducing conditions.

      Exposure time : 10 seconds

    References for WISP2 protein (Active) (ab50040)

    ab50040 has not yet been referenced specifically in any publications.

    Publishing research using ab50040 ? Please let us know so that we can cite the reference in this datasheet

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"