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Products:Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Overview
Product name
Anti-WNK1 antibody
See all WNK1 products (4) ...
Description
Rabbit polyclonal to WNK1
Tested applications
WB, ELISA, IHC-P, IHC-Frmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthesized non-phosphopeptide derived from huma WNK1 around the phosphorylation site of threonine 58 (R-H-T-M-D).
Positive control
EGF-treated 293 cell extract; human breast carcinoma tissue.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Applications
Show applications key
Our Abpromise guarantee covers the use of ab53151 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/300 - 1/1000.Predicted molecular weight: 251 kDa.
ELISA
ELISA: 1/10000ELISA: 1/10000
IHC-P: 1/50 - 1/100.
IHC-Fr: Use at an assay dependent dilution. (PubMed: 20398666)
Target
Function
Controls sodium and chloride ion transport by inhibiting the activity of WNK4, potentially by either phosphorylating the kinase or via an interaction between WNK4 and the autoinhibitory domain of WNK1. WNK4 regulates the activity of the thiazide-sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation. WNK1 may also play a role in actin cytoskeletal reorganization.
Tissue specificity
Widely expressed, with highest levels observed in the testis, heart, kidney and skeletal muscle. Isoform 3 is kidney-specific.
Involvement in disease
Defects in WNK1 are a cause of pseudohypoaldosteronism type II (PHAII) [MIM:145260]. PHAII is an autosomal dominant disease characterized by severe hypertension, hyperkalemia, and sensitivity to thiazide diuretics which may result from a chloride shunt in the renal distal nephron.
Defects in WNK1 are a cause of hereditary sensory and autonomic neuropathy type 2A (HSAN2A) [MIM:201300]. A form of hereditary sensory and autonomic neuropathy, a genetically and clinically heterogeneous group of disorders characterized by degeneration of dorsal root and autonomic ganglion cells, and by sensory and/or autonomic abnormalities. HSAN2A is an autosomal recessive disorder characterized by impairment of pain, temperature and touch sensation, onset of symptoms in infancy or early childhood, occurrence of distal extremity pathologies (paronychia, whitlows, ulcers, and Charcot joints), frequent amputations, sensory loss that affects all modalities of sensation (lower and upper limbs and perhaps the trunk as well), absence or diminution of tendon reflexes (usually in all limbs), minimal autonomic dysfunction, absence of sensory nerve action potentials, and virtual absence of myelinated fibers with decreased numbers of unmyelinated fibers in sural nerves.
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WNK subfamily.
Contains 1 protein kinase domain.
Post-translational
modifications
O-glycosylated.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionQ9H4A3
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- MGC163341 antibody
- Erythrocyte 65 kDa protein antibody
- HSAN2 antibody
see all
Database links
- Entrez Gene: 65125 Human
- Entrez Gene: 100503989 Mouse
- Entrez Gene: 232341 Mouse
- Entrez Gene: 116477 Rat
- Omim: 605232 Human
- SwissProt: Q4VBX9 Human
- SwissProt: Q9H4A3 Human
- SwissProt: P83741 Mouse
see all
Anti-WNK1 antibody images:
Immunohistochemistry (Paraffin-embedded sections) - WNK1 antibody (ab53151)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with ab53151 at a dilution of 1/50-1/100. Right hand image: treated with immunising peptide. Left hand image: untreated.
Western blot - WNK1 antibody (ab53151)
All lanes : Anti-WNK1 antibody (ab53151) at 1/300 dilution
Lane 1 : Extracts from 293 cells treated with EGF (200ng/ml, 30 min) with no peptide
Lane 2 : Extracts from 293 cells treated with EGF (200ng/ml, 30 min) with peptide
Predicted band size : 251 kDa
References for Anti-WNK1 antibody (ab53151)
This product has been referenced in:
- Park HWet al. Dynamic regulation of CFTR bicarbonate permeability by [Cl-]i and its role in pancreatic bicarbonate secretion. Gastroenterology 139:620-31 (2010). IHC-Fr; Human.Read more (PubMed: 20398666) »
See 1 publication for this product
Publishing research using ab53151? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"