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Immunohistochemistry (Paraffin-embedded sections) - WNK1 antibody (ab53151)
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Western blot - WNK1 antibody (ab53151)
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Product Name
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WNK1 antibody
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See all WNK1 antibodies (5)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to WNK1
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Immunogen
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Synthesized non-phosphopeptide derived from huma WNK1 around the phosphorylation site of threonine 58 (R-H-T-M-D).
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Reacts with
(species key)
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Hu, Ms, Rat
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Tested applications
(see key)
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ELISA, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/10000.
IHC-P: 1/50 - 1/100.
WB: 1/300 - 1/1000. Predicted molecular weight: 251 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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EGF-treated 293 cell extract; human breast carcinoma tissue.
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Cellular localization
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Cytoplasmic
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Research areas
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Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
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Relevance
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WNK1 controls sodium and chloride ion transport by inhibiting the activity of WNK4, potentially by either phosphorylating the kinase or via an interaction between WNK4 and the autoinhibitory domain of WNK1. WNK4 regulates the activity of the thiazide sensitive Na/Cl cotransporter, SLC12A3, by phosphorylation. WNK1 may also play a role in actin cytoskeletal reorganization.
WNK1 has 4 isoforms produced by alternative splicing. WNK1 is widely expressed, with highest levels observed in the testis, heart, kidney and skeletal muscle.
Defects in WNK1 are a cause of pseudohypoaldosteronism type II (PHAII), an autosomal dominant disease characterized by severe hypertension, hyperkalemia, and sensitivity to thiazide diuretics which may result from a chloride shunt in the renal distal nephron.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at -20°C. Stable for 12 months at -20°C
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this WNK1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for WNK1 antibody images, references, products related to ab53151 and other tools.
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WNK1 antibody images:
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 Immunohistochemistry (Paraffin-embedded sections) - WNK1 antibody (ab53151)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with ab53151 at a dilution of 1/50-1/100. Right hand image: treated with immunising peptide. Left hand image: untreated.
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Western blot - WNK1 antibody (ab53151)
All lanes : WNK1 antibody (ab53151) at 1/300 dilution
Lane 1 : Extracts from 293 cells treated with EGF (200ng/ml, 30 min) with no peptide
Lane 2 : Extracts from 293 cells treated with EGF (200ng/ml, 30 min) with peptide
Predicted band size : 251 kDa
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Search PubMed (MEDLINE) for references to WNK1
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WNK1 antibody - more information
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Customer reviews (feedback) regarding WNK1 antibody |
Customer FAQs regarding WNK1 antibody |
Protocols for WNK1 antibody |
Price and availability of products related to WNK1 antibody |
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WNK1 antibody - related products:
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WNK1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about WNK1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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