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Products:Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Overview
Product name
Anti-WNK1 (phospho T58) antibody
See all WNK1 products (4) ...
Description
Rabbit polyclonal to WNK1 (phospho T58)
Specificity
ab53137 detects endogenous levels of WNK1 only when phosphorylated at threonine 58.
Tested applications
WB, IHC-P, ELISAmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
A synthetic phosphopeptide derived from human WNK1 around the phosphorylation site of threonine 58 (R-H-TP-M-D).
Positive control
293 cell extracts treated with EGF (200ng/ml, 30mins) and human breast carcinoma tissue.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab53137 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Applications
Show applications key
Our Abpromise guarantee covers the use of ab53137 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/300 - 1/1000.Detects a band of approximately 251 kDa (predicted molecular weight: 251 kDa).
IHC-P: 1/50 - 1/100.
ELISA
ELISA: 1/5000ELISA: 1/5000
Target
Function
Controls sodium and chloride ion transport by inhibiting the activity of WNK4, potentially by either phosphorylating the kinase or via an interaction between WNK4 and the autoinhibitory domain of WNK1. WNK4 regulates the activity of the thiazide-sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation. WNK1 may also play a role in actin cytoskeletal reorganization.
Tissue specificity
Widely expressed, with highest levels observed in the testis, heart, kidney and skeletal muscle. Isoform 3 is kidney-specific.
Involvement in disease
Defects in WNK1 are a cause of pseudohypoaldosteronism type II (PHAII) [MIM:145260]. PHAII is an autosomal dominant disease characterized by severe hypertension, hyperkalemia, and sensitivity to thiazide diuretics which may result from a chloride shunt in the renal distal nephron.
Defects in WNK1 are a cause of hereditary sensory and autonomic neuropathy type 2A (HSAN2A) [MIM:201300]. A form of hereditary sensory and autonomic neuropathy, a genetically and clinically heterogeneous group of disorders characterized by degeneration of dorsal root and autonomic ganglion cells, and by sensory and/or autonomic abnormalities. HSAN2A is an autosomal recessive disorder characterized by impairment of pain, temperature and touch sensation, onset of symptoms in infancy or early childhood, occurrence of distal extremity pathologies (paronychia, whitlows, ulcers, and Charcot joints), frequent amputations, sensory loss that affects all modalities of sensation (lower and upper limbs and perhaps the trunk as well), absence or diminution of tendon reflexes (usually in all limbs), minimal autonomic dysfunction, absence of sensory nerve action potentials, and virtual absence of myelinated fibers with decreased numbers of unmyelinated fibers in sural nerves.
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WNK subfamily.
Contains 1 protein kinase domain.
Post-translational
modifications
O-glycosylated.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionQ9H4A3
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- MGC163341 antibody
- Erythrocyte 65 kDa protein antibody
- HSAN2 antibody
see all
Database links
- Entrez Gene: 65125 Human
- Entrez Gene: 100503989 Mouse
- Entrez Gene: 232341 Mouse
- Entrez Gene: 116477 Rat
- Omim: 605232 Human
- SwissProt: Q4VBX9 Human
- SwissProt: Q9H4A3 Human
- SwissProt: P83741 Mouse
see all
Anti-WNK1 (phospho T58) antibody images:
Western blot - WNK1 (phospho T58) antibody (ab53137)
All lanes : Anti-WNK1 (phospho T58) antibody (ab53137) at 1/300 dilution
Lane 1 : 293 cell extract treated with EGF (200ng/ml, 30mins)
Lane 2 : 293 cell extract treated with EGF (200ng/ml, 30mins) with immunising peptide
Predicted band size : 251 kDa
Immunohistochemistry (Paraffin-embedded sections) - WNK1 (phospho T58) antibody (ab53137)
ab53137 at 1/50 dilution staining WNK1 in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.
References for Anti-WNK1 (phospho T58) antibody (ab53137)
This product has been referenced in:
- Cheng CJ & Huang CL Activation of PI3-Kinase Stimulates Endocytosis of ROMK via Akt1/SGK1-Dependent Phosphorylation of WNK1. J Am Soc Nephrol 22:460-71 (2011). WB; Human.Read more (PubMed: 21355052) »
See 1 publication for this product
Publishing research using ab53137? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"