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- Proteins and Peptides
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Buying a fast track?
We are able to bring you this product at a low price as it is not yet fully characterized. Our Scientific Support team are available to assist you, but we cannot offer refunds or replacements on this product.
What is a fast track? »Overview
Product name
Anti-WNK2 antibody
See all WNK2 products (2) ...
Description
Rabbit polyclonal to WNK2
This product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »
Cross reactivity
Predicted to work with
Human
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1800 - 1900 of Human WNK2.
(Peptide available as ab306 98.)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
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Western blot - WNK2 antibody (ab28852)
(enlarge)
Applications
Show applications key
Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA).Fast track terms of use
WB: 1/500. Detects a band of approximately 300 kDa (predicted molecular weight: 300 kDa).Lysates from transfected HEK293 cells expressing Myc-WNK2 as well as endogenous WNK2
ELISA: Use at an assay dependent dilution. This antibody gave a positive result in ELISA against the immunizing peptide (ab30698).
Target
Relevance
WNK2 a putative tumour suppressor is a member of a family of serine / threonine protein kinases implicated in the hereditary form of hypertension, pseudohypoaldosteronism type II.
Cellular localization
Nuclear
Alternative names
- PRKWNK2 antibody
- Protein kinase lysine deficient 2 antibody
- Protein kinase with no lysine 2 antibody
- Serine/Threonine protein kinase WNK2 antibody
Database links
- Entrez Gene: 65268 Human
- Omim: 606249 Human
- SwissProt: Q9Y3S1 Human
- Unigene: 522291 Human
Anti-WNK2 antibody images:
This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.
Western blot - WNK2 antibody (ab28852)
All lanes : Top blot: anti Myc-Tag antibody; Bottom Blot: ab28852 (1/500)
Lane 1 : Hek293 cell lysate Transfected with empty vector
Lane 2 : Hek293 cell lysate Transfected with MYC-WNK1
Lane 3 : Hek293 cell lysate Transfected with MYC-WNK2
Lane 4 : Hek293 cell lysate Transfected with MYC-WNK3
Lane 5 : Hek293 cell lysate Transfected with MYC-WKN4
Secondary
Goat anti-rabbit IgG conjugated to HRP at 1/3000 dilution
developed using the ECL technique
Predicted band size : 300 kDa
Anti-tubulin antibody used as loading control for both blots.
image courtesy to abreview from Peter Jordan
References for Anti-WNK2 antibody (ab28852)
ab28852 has not yet been referenced specifically in any publications.
Publishing research using ab28852? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"