WNK4 antibody (ab52847)

Product Name 

WNK4 antibody

Product type 

Primary antibodies

Description 

Mouse polyclonal to WNK4

Immunogen 

Fusion protein: FLDASDPALQ PPGGLPSSPA ESHLCLPSGF ALSIPRSGPG SDFSPGDSYA SDAASGLSDM GEGGQMRKNP VKTLRRRPRS RLRVTSVSDQ SDRVVECQLQ , corresponding to amino acids 591-690 of Mouse WNK4; which is tagged.
BLAST 'FLDASDPALQ PPGGLPSSPA ESHLCLPSGF ALSIPRSGPG SDFSPGDSYA SDAASGLSDM GEGGQMRKNP VKTLRRRPRS RLRVTSVSDQ SDRVVECQLQ ' with or

Reacts with 

Ms

Predicted to react 
(species key)

Rat
(Due to sequence homology)

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
WB: 1/1000. Predicted molecular weight: 135 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cell junctions; tight junctions

Research areas 

Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases

Relevance 

WNK4 appears to act as a molecular switch that can vary the balance between sodium chloride reabsorption and K(+) secretion to maintain integrated homeostasis. It regulates the activity of the thiazide-sensitive Na-Cl cotransporter SLC12A3 by phosphorylation, which appears to prevent membrane trafficking of SLC12A3. It also inhibits the renal K(+) channel KCNJ1 via a kinase independent mechanism, by which it induces clearance of the protein from the cell surface by clathrin dependent endocytosis.

This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

Database links 

Raised in 

Mouse

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Whole antiserum

Storage buffer 

Preservative: None
Constituents: 50% Glycerol, Whole serum

Material safety datasheet (MSDS) for this product:
Glycerol MSDS

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

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