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Overview

  • Product nameWSB1 protein (Tagged-His Tag)See all WSB1 proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 26-242 (217 amino acids) of Human WSB1 with N terminal His tag; MWt 25kDa (UniProt Q9Y6I7).
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 96 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Additional notesProtein Identity confirmed by Mass Spectrometry (MS/MS) (acquired on initial reference batch)
  • Sequence notesAAPFDKKCGRENWTVAFAPDGSYFAWSQGHRTVKLVPWSQ CLQNFLLHG TKNVTNSSSLRLPRQNSDGGQKNKPREHI IDCGDIVWSLAFGSSVPEKQ SRCVNIEWHRFRFGQDQL LLATGLNNGRIKIWDVYTGKLLLNLVDHTEV VRDLTFA PDGSLILVSASRDKTLRVWDLKDDGNMMKVLRGHQNWVYS CA FSPDSSMLCSVGASKAVFLWN
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab92160 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    MS
    SDS-PAGE
  • Application notesMS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        SOCS box containing WD protein SWiP 1SWIP1WD repeat and SOCS box containing 1
        WD repeat and SOCS box containing protein 1WSB 1
      see all
  • RelevanceWSB1 is a member of the WD-protein subfamily. It shares a high sequence identity to mouse and chick proteins. It contains six WD-repeats spanning most of the protein and an SOCS box in the C-terminus. It is a probable substrate-recognition component of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. It recognizes type II iodothyronine deiodinase/DIO2. There are two named isoforms.
  • Cellular localizationIntracellular

    • WSB1 protein (Tagged-His Tag) images

    • SDS-PAGE - WSB1 protein (Tagged-His Tag) (ab92160)
      SDS-PAGE - WSB1 protein (Tagged-His Tag) (ab92160)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for WSB1 protein (Tagged-His Tag) (ab92160)

    ab92160 has not yet been referenced specifically in any publications.

    Publishing research using ab92160 ? Please let us know so that we can cite the reference in this datasheet

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"