Anti-WSTF antibody (ab70263)
Key features and details
- Rabbit polyclonal to WSTF
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-WSTF antibody
See all WSTF primary antibodies -
Description
Rabbit polyclonal to WSTF -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Opossum, Orangutan -
Immunogen
A region between residues 1 and 50 of Human WSTF (SwissProt entry Q9UIG0)
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab70263 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 200 kDa (predicted molecular weight: 171 kDa).
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IP |
Use at 2-5 µg/mg of lysate.
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 200 kDa (predicted molecular weight: 171 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Atypical tyrosine-protein kinase that plays a central role in chromatin remodeling and acts as a transcription regulator. Involved in DNA damage response by phosphorylating 'Tyr-142' of histone H2AX (H2AXY142ph). H2AXY142ph plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Essential component of the WICH complex, a chromatin remodeling complex that mobilizes nucleosomes and reconfigures irregular chromatin to a regular nucleosomal array structure. The WICH complex regulates the transcription of various genes, has a role in RNA polymerase I and RNA polymerase III transcription, mediates the histone H2AX phosphorylation at 'Tyr-142', and is involved in the maintenance of chromatin structures during DNA replication processes. In the complex, it mediates the recruitment of the WICH complex to replication foci during DNA replication. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. In the WINAC complex, plays an essential role by targeting the complex to acetylated histones, an essential step for VDR-promoter association. -
Tissue specificity
Ubiquitously expressed with high levels of expression in heart, brain, placenta, skeletal muscle and ovary. -
Involvement in disease
Note=BAZ1B is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of BAZ1B may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease. -
Sequence similarities
Belongs to the WAL family. BAZ1B subfamily.
Contains 1 bromo domain.
Contains 1 DDT domain.
Contains 1 PHD-type zinc finger.
Contains 1 WAC domain. -
Developmental stage
Expressed at equal levels in 19-23 weeks old fetal tissues. -
Domain
The N-terminal part (1-345), including the WAC domain and the C motif, mediates the tyrosine-protein kinase activity.
The bromo domain mediates the specific interaction with acetylated histones. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. Accumulates in pericentromeric heterochromatin during replication. Targeted to replication foci throughout S phase via its association with PCNA. - Information by UniProt
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Database links
- Entrez Gene: 9031 Human
- Entrez Gene: 368002 Rat
- Omim: 605681 Human
- SwissProt: Q9UIG0 Human
- Unigene: 647016 Human
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Alternative names
- baz1b antibody
- BAZ1B_HUMAN antibody
- Bromodomain adjacent to zinc finger domain protein 1B antibody
see all
Images
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All lanes : Anti-WSTF antibody (ab70263) at 0.05 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 5 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 50 µg
Predicted band size: 171 kDa
Observed band size: 200 kDa why is the actual band size different from the predicted?
Additional bands at: 140 kDa, 190 kDa, 300 kDa. We are unsure as to the identity of these extra bands. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placenta tissue labelling WSTF with ab70263 at 1/200 (1µg/ml). Detection: DAB.
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1 mg of whole cell lysate from HeLa cells was immunoprecipitated using ab70263 at 3ug/mg lysate (lane 1) or a control rabbit Ig (lane 2). The subsequent blot was carried with ab70263 at 0.05ug/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab70263 has been referenced in 1 publication.
- Jiang D et al. Williams syndrome transcription factor promotes proliferation and invasion of cervical cancer cells by regulating PI3K/Akt signaling pathway. J Obstet Gynaecol Res 47:2433-2441 (2021). PubMed: 34028125