Anti-WWOX antibody (ab33248)

I prefer the credit note, does it means that I can order other antibody with this credit note from your company? How do you give me
the credit not?

ANSWER:

Thank you for your reply.
Your credit note ID is *****.
I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a future order by passing it on to your purchasing department. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. If you have questions on how to use the credit note, please contact our accounting department.
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The credit note ID is for your reference only and does not automatically guarantee the credit.
I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

LOT NUMBER GR21670-5
ORDER NUMBER
DESCRIPTION OF THE PROBLEM very weak signal with several hours exposure or sometime no signal at all
SAMPLE MCF-7， MB175， MB415 ETC cell extract
PRIMARY ANTIBODY WWOX(ab33248) 1:500 4℃ 12h wash with TBST 6min X 3 times
DETECTION METHOD ECL exposure for several hours
POSITIVE AND NEGATIVE CONTROLS USED GAPDH AND PTEN as positive control
ANTIBODY STORAGE CONDITIONS -20℃
SAMPLE PREPARATION RIPA with protease inhibitor(leupeptin, PMSF ect) Heating: 100℃ 7min
AMOUNT OF PROTEIN LOADED 80ug
ELECTROPHORESIS/GEL CONDITIONS 10% Reducing gel
TRANSFER AND BLOCKING CONDITIONS 3.9mM Glycine, 4.8mM Tris and 0.037%SDS 360mA for 2h Blocking with 5% milk(BD 232100)
SECONDARY ANTIBODY goat anti-rabbit IgG-HRP: sc-2004 1:1000 RT 2h wash with TBST 6min X 3 times
HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 5
HAVE YOU RUN A "NO PRIMARY" CONTROL? Yes
DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes
WHAT STEPS HAVE YOU ALTERED? Primary antibody's dilution(1: 500, 1:1000, 1:2000) secondary antibody's dilution(1:1000, 1:2000, 1:5000)

ANSWER:

Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hear this antibody is not providing satisfactory results.
The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful.
Having reviewed the protocol details, I believe this product should have given satisfactory results. It appears that you may have received a faulty vial.
I apologize for the inconvenience and am pleased to offer you in compensation a credit note, a refund, or free of charge replacement with an alternative anti-WWOX such as :
ab58587, www.abcam.com/ab58587
ab42664, www.abcam.com/ab42664
ab78210, www.abcam.com/ab78210
I am not offering a replacement with another vial of ab33248 as the only lot we have in stock is the same as the one you received originally.
Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed.