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Products:Cell Biology >> Apoptosis >> Intracellular >> Associated Proteins
Overview
Product name
Anti-WWOX antibody
See all WWOX products (6) ...
Description
Rabbit polyclonal to WWOX
Specificity
ab33248 recognises WWOX.
Tested applications
WB, IHC-P, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Rat, Cow, Dog, Chimpanzee
Immunogen
Synthetic peptide: RLAFTVDDNPTKPTTRQRY, corresponding to amino acids 89/107 of Human WWOX
RLAFTVDDNP TKPTTRQRY
Positive control
L929 cells, human skin tissue.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> p53 pathway
Cancer >> Growth factors >> TNF
Cancer >> Growth factors >> TGF
Epigenetics and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Tumor Suppressors
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
Cell Biology >> Apoptosis >> Intracellular >> Associated Proteins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab33248 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000 - 1/5000.Predicted molecular weight: 47 kDa.
IHC-P: 1/1000Antigen retrieval is not essential but may optimise staining.
ICC/IF: 1/1000
Target
Function
Putative oxidoreductase. Acts as a tumor suppressor and plays a role in apoptosis. Required for normal bone development (By similarity). May function synergistically with p53/TP53 to control genotoxic stress-induced cell death. Plays a role in TGFB1 signaling and TGFB1-mediated cell death. May also play a role in tumor necrosis factor (TNF)-mediated cell death. Inhibits Wnt signaling, probably by sequestering DVL2 in the cytoplasm.
Tissue specificity
Widely expressed. Strongly expressed in testis, prostate, and ovary. Overexpressed in cancer cell lines. Isoform 5 and isoform 6 may only be expressed in tumor cell lines.
Involvement in disease
Note=Defects in WWOX may be involved in several cancer types. The gene spans the second most common chromosomal fragile site (FRA16D) which is frequently altered in cancers. Alteration of the expression and expression of some isoforms is associated with cancers. However, it is still unclear if alteration of WWOX is directly implicated in cancerogenesis or if it corresponds to a secondary effect.
Defects in WWOX may be a cause of esophageal cancer (ESCR) [MIM:133239].
Sequence similarities
Belongs to the short-chain dehydrogenases/reductases (SDR) family.
Contains 2 WW domains.
Domain
The WW 1 domain mediates interaction with TP53, and probably TP73, TFAP2C, LITAF and WBP1.
Post-translational
modifications
Phosphorylated upon genotoxic stress. Phosphorylation of Tyr-33 regulates interaction with TP53, TP73 and MAPK8. May also regulate proapoptotic activity. Phosphorylation by TNK2 is associated with polyubiquitination and degradation.
Ubiquitinated when phosphorylated by TNK2, leading to its degradation.
Cellular localization
Cytoplasm. Nucleus. Mitochondrion. Golgi apparatus. Partially localizes to the mitochondria. Translocates to the nucleus upon genotoxic stress or TNF stimulation (By similarity). Translocates to the nucleus in response to TGFB1. Isoform 5 and isoform 6 may localize in the nucleus.
Target information above from: UniProt accessionQ9NZC7
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 5330426P09Rik antibody
- 9030416C10Rik antibody
- D16S432E antibody
see all
Database links
- Entrez Gene: 51741 Human
- Entrez Gene: 80707 Mouse
- Entrez Gene: 292041 Rat
- Omim: 605131 Human
- SwissProt: Q9NZC7 Human
- SwissProt: Q91WL8 Mouse
- Unigene: 461453 Human
- Unigene: 440420 Mouse
Anti-WWOX antibody images:
Western blot - WWOX antibody (ab33248)
All lanes : Anti-WWOX antibody (ab33248) at 1/5000 dilution
Lane 1 : murine fibroblast L929 cell lysate
Lane 2 : murine fibroblast L929 cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size : 47 kDa
Observed band size : 47 kDa
Lanes 1 and 2 are duplicates.
Immunohistochemistry (Formalin-fixed paraffin-embedded sections) - WWOX antibody (ab33248)
ab33248, at a 1/1000 dilution, staining human WWOX in human skin tissue by Immunohistochemistry, Formalin fixed Paraffin embedded tissue
Immunofluorescence - WWOX antibody (ab33248)
These images show a L929 cell expressing WWOX (green, 1/1000 dilution) with DAPI staining (blue) for nucleus.
References for Anti-WWOX antibody (ab33248)
ab33248 has not yet been referenced specifically in any publications.
Publishing research using ab33248? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"