|
|
|
|
Western blot - WWOX antibody (ab42664)
(enlarge)
|
|
Immunocytochemistry/ Immunofluorescence - WWOX antibody (ab42664)
(enlarge)
|
|
|
|
|
Product Name
|
WWOX antibody
|
| |
See all WWOX antibodies (7)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rabbit polyclonal to WWOX
|
|
Immunogen
|
Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human WWOX. (Peptide available as ab45233.)
|
|
Reacts with
|
Hu
|
|
Predicted to react
(species key)
|
Ms, Cow, Orngt
(Due to sequence homology)
|
|
Tested applications
(see key)
|
ICC/IF, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
ICC/IF: Use at a concentration of 1 µg/ml.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Positive control
(see definition)
|
ab42664 gave a positive result in Human Ovary Tissue Lysate.
|
|
Cellular localization
|
Cytoplasmic, Golgi Apparatus, Mitochondrial and Nuclear
|
|
Research areas
|
Chromatin and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Tumor Suppressors
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
Cell Biology >> Apoptosis >> Intracellular >> Associated Proteins
|
|
Relevance
|
WW domain-containing oxidoreductase WWOX, also known as WOX1 or FOR, is a proapoptotic protein and a tumor suppressor gene. Because mutation or deletion in the coding region of WWOX is rarely found, it is speculated that the appearance of aberrant transcripts affects progression of many cancers. In addition, protein levels of WWOX, its Tyr33-phosphorylated form, and WOX2 are significantly down-regulated in the neurons of Alzheimer's hippocampi.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Rabbit
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
Immunogen affinity purified
|
|
Storage buffer
|
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
|
|
Form
|
Liquid
|
|
Concentration
|
0.500 mg/ml
|
|
Storage instructions
|
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this WWOX antibody is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for WWOX antibody images, references, products related to ab42664 and other tools.
|
| |
WWOX antibody images:
|
 Western blot - WWOX antibody (ab42664)
All lanes : WWOX antibody (ab42664) at 1 µg/ml
Lane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)
Lane 2 : Ovary (Human) Tissue Lysate - adult normal tissue (ab30222)
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 47 kDa
Observed band size : 47 kDa
Additional bands at : 22 kDa (possible isoform),41 kDa (possible isoform).
WWOX has 7 isoforms. ab42664 recognises WWOX (47 kDa) and possibly WWOX2 (41 kDa) in human ovary tissue. It appears that this antibody also recognises WWOX3 (22 kDa) in human brain tissue.
|
| |
Immunocytochemistry/ Immunofluorescence - WWOX antibody (ab42664)
ICC/IF image of ab42664 stained human HeLa cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab42664, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HEK 293, HepG2 and MCF7 cells.
|
| |
| |
|
Search PubMed (MEDLINE) for references to WWOX
|
| |
WWOX antibody - more information
|
Customer reviews (feedback) regarding WWOX antibody |
Customer FAQs regarding WWOX antibody |
Protocols for WWOX antibody |
Price and availability of products related to WWOX antibody |
| |
WWOX antibody - related products:
|
|
|
| |
WWOX antibody - other tools:
|
Contact Abcam with a Technical Enquiry about WWOX antibody |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
