Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab42664 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).Can be blocked with Human WWOX peptide (ab45233).

Target

  • FunctionPutative oxidoreductase. Acts as a tumor suppressor and plays a role in apoptosis. Required for normal bone development (By similarity). May function synergistically with p53/TP53 to control genotoxic stress-induced cell death. Plays a role in TGFB1 signaling and TGFB1-mediated cell death. May also play a role in tumor necrosis factor (TNF)-mediated cell death. Inhibits Wnt signaling, probably by sequestering DVL2 in the cytoplasm.
  • Tissue specificityWidely expressed. Strongly expressed in testis, prostate, and ovary. Overexpressed in cancer cell lines. Isoform 5 and isoform 6 may only be expressed in tumor cell lines.
  • Involvement in diseaseNote=Defects in WWOX may be involved in several cancer types. The gene spans the second most common chromosomal fragile site (FRA16D) which is frequently altered in cancers. Alteration of the expression and expression of some isoforms is associated with cancers. However, it is still unclear if alteration of WWOX is directly implicated in cancerogenesis or if it corresponds to a secondary effect.
    Defects in WWOX may be a cause of esophageal cancer (ESCR) [MIM:133239].
  • Sequence similaritiesBelongs to the short-chain dehydrogenases/reductases (SDR) family.
    Contains 2 WW domains.
  • DomainThe WW 1 domain mediates interaction with TP53, and probably TP73, TFAP2C, LITAF and WBP1.
  • Post-translational
    modifications
    Phosphorylated upon genotoxic stress. Phosphorylation of Tyr-33 regulates interaction with TP53, TP73 and MAPK8. May also regulate proapoptotic activity. Phosphorylation by TNK2 is associated with polyubiquitination and degradation.
    Ubiquitinated when phosphorylated by TNK2, leading to its degradation.
  • Cellular localizationCytoplasm. Nucleus. Mitochondrion. Golgi apparatus. Partially localizes to the mitochondria. Translocates to the nucleus upon genotoxic stress or TNF stimulation (By similarity). Translocates to the nucleus in response to TGFB1. Isoform 5 and isoform 6 may localize in the nucleus.
  • Target information above from: UniProt accession Q9NZC7 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • 5330426P09Rik antibody
    • 9030416C10Rik antibody
    • Aberrant WW domain-containing oxidoreductase antibody
    • D16S432E antibody
    • EC 1.1.1.- antibody
    • FOR antibody
    • FOR II protein antibody
    • FRA16D antibody
    • fragile site FRA16D oxidoreductase antibody
    • Fragile site FRA16D Oxireductase antibody
    • HHCMA56 antibody
    • MGC55975 antibody
    • PRO0128 antibody
    • putative oxidoreductase antibody
    • SDR41C1 antibody
    • Short chain dehydrogenase/reductase family 41C, member 1 antibody
    • WOX1 antibody
    • WW domain containing oxidoreductase antibody
    • WW domain-containing oxidoreductase antibody
    • WW domain-containing protein WWOX antibody
    • wwox antibody
    • WWOX_HUMAN antibody
    • zgc:55975 antibody
    see all

Anti-WWOX antibody images

  • All lanes : Anti-WWOX antibody (ab42664) at 1 µg/ml

    Lane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)
    Lane 2 : Ovary (Human) Tissue Lysate - adult normal tissue (ab30222)

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 47 kDa
    Observed band size : 47 kDa
    Additional bands at : 22 kDa (possible isoform),41 kDa (possible isoform).
  • ICC/IF image of ab42664 stained human HeLa cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab42664, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HEK 293, HepG2 and MCF7 cells.

References for Anti-WWOX antibody (ab42664)

ab42664 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Brain sections)
Specification Brain sections
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Username

Dr. Sophie Pezet

Verified customer

Submitted Jan 28 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"