Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Cell Biology >> Apoptosis >> Intracellular >> Associated Proteins
Overview
Product name
Anti-WWOX (phospho Y33) antibody
See all WWOX products (6) ...
Description
Rabbit polyclonal to WWOX (phospho Y33)
Specificity
This antibody recognizes the ~46 kDa WWOXv1 and the ~41 kDa WWOXv2 proteins phosphorylated at Tyr33. It may recognize other expressed isoforms of WWOX (WWOXv3-WWOXv8).
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Predicted to work with
Chicken, Cow, Orangutan
Immunogen
A synthetic phosphopeptide (KDGWVpYYANHTEEKT) surrounding the Tyr33 phosphorylation site in mouse WWOX1
KDGWVpYYAN HTEEKT
Positive control
SK-N-SH cells treated with anisomycin
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: Whole serum
Purity
Whole antiserum
Purification notes
Rabbit polyclonal antibody supplied as undiluted serum.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> p53 pathway
Cancer >> Growth factors >> TNF
Cancer >> Growth factors >> TGF
Epigenetics and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Tumor Suppressors
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
Cell Biology >> Apoptosis >> Intracellular >> Associated Proteins
-
Immunohistochemistry (Paraffin-embedded sections) - WWOX (phospho Y33) antibody (ab58587)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab58587 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000 - 1/3000.Predicted molecular weight: 47 kDa.
IHC-P: 1/200
Target
Function
Putative oxidoreductase. Acts as a tumor suppressor and plays a role in apoptosis. Required for normal bone development (By similarity). May function synergistically with p53/TP53 to control genotoxic stress-induced cell death. Plays a role in TGFB1 signaling and TGFB1-mediated cell death. May also play a role in tumor necrosis factor (TNF)-mediated cell death. Inhibits Wnt signaling, probably by sequestering DVL2 in the cytoplasm.
Tissue specificity
Widely expressed. Strongly expressed in testis, prostate, and ovary. Overexpressed in cancer cell lines. Isoform 5 and isoform 6 may only be expressed in tumor cell lines.
Involvement in disease
Note=Defects in WWOX may be involved in several cancer types. The gene spans the second most common chromosomal fragile site (FRA16D) which is frequently altered in cancers. Alteration of the expression and expression of some isoforms is associated with cancers. However, it is still unclear if alteration of WWOX is directly implicated in cancerogenesis or if it corresponds to a secondary effect.
Defects in WWOX may be a cause of esophageal cancer (ESCR) [MIM:133239].
Sequence similarities
Belongs to the short-chain dehydrogenases/reductases (SDR) family.
Contains 2 WW domains.
Domain
The WW 1 domain mediates interaction with TP53, and probably TP73, TFAP2C, LITAF and WBP1.
Post-translational
modifications
Phosphorylated upon genotoxic stress. Phosphorylation of Tyr-33 regulates interaction with TP53, TP73 and MAPK8. May also regulate proapoptotic activity. Phosphorylation by TNK2 is associated with polyubiquitination and degradation.
Ubiquitinated when phosphorylated by TNK2, leading to its degradation.
Cellular localization
Cytoplasm. Nucleus. Mitochondrion. Golgi apparatus. Partially localizes to the mitochondria. Translocates to the nucleus upon genotoxic stress or TNF stimulation (By similarity). Translocates to the nucleus in response to TGFB1. Isoform 5 and isoform 6 may localize in the nucleus.
Target information above from: UniProt accessionQ9NZC7
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 5330426P09Rik antibody
- 9030416C10Rik antibody
- D16S432E antibody
see all
Database links
- Entrez Gene: 51741 Human
- Entrez Gene: 80707 Mouse
- Entrez Gene: 292041 Rat
- Omim: 605131 Human
- SwissProt: Q9NZC7 Human
- SwissProt: Q91WL8 Mouse
- Unigene: 461453 Human
- Unigene: 440420 Mouse
Anti-WWOX (phospho Y33) antibody images:
Immunohistochemistry (Paraffin-embedded sections) - WWOX (phospho Y33) antibody (ab58587)
Detection of WWOX by staining paraffin sections.
Sample: Breast ductal carcinoma.
Primary antibody: ab58587 (1/200), Anti-COX4, and DAPI as indicated.
Detection: fluorescence.
References for Anti-WWOX (phospho Y33) antibody (ab58587)
ab58587 has not yet been referenced specifically in any publications.
Publishing research using ab58587? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"