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Product name
Anti-WWP1 antibody
See all WWP1 products (3) ...
Description
Rabbit polyclonal to WWP1
Tested applications
ICC/IF, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Cow
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human WWP1.
(Peptide available as ab437 90.)
Positive control
This antibody gave a positive signal in the following whole cell lysates: MCF7 HeLa
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Tumor biomarkers >> Other
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> Other E3 Ligases
Applications
Show applications key
Our Abpromise guarantee covers the use of ab43791 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 5 µg/ml.
WB: Use a concentration of 1 µg/ml. Detects a band of approximately 105 kDa (predicted molecular weight: 105 kDa).
Target
Relevance
WWP1 is an E3 ubiquitin ligase and belongs to a family of NEDD4-like proteins. WWP1 contains 4 tandem WW domains and a HECT (homologous to the E6-associated protein carboxyl terminus) domain. WW domain-containing proteins are found in all eukaryotes and play an important role in the regulation of a wide variety of cellular functions such as protein degradation, transcription, and RNA splicing. The HECT domain of WWP1 has been implicated in regulating the localization and stability of p53 – inhibition of WWP1 results in a decrease in p53 expression, whilst WWP1 mediated stabilization of p53 appears to be associated with an accumulation of cytoplasmic p53. WWP1 also negatively regulates the TGF beta tumor suppressor pathway by inactivating its molecular components (SMAD2, SMAD4 and TGFbetaR1). WWP1 has been implicated in both breast and prostate cancers.
Cellular localization
Cytoplasmic, Nuclear and Plasma membrane
Alternative names
- AIP 5 antibody
- AIP5 antibody
- Atropin-1-interacting protein 5 antibody
see all
Database links
- Entrez Gene: 11059 Human
- Entrez Gene: 107568 Mouse
- Entrez Gene: 297930 Rat
- Omim: 602307 Human
- SwissProt: Q9H0M0 Human
- SwissProt: Q8BZZ3 Mouse
- SwissProt: Q3UV52 Mouse
- SwissProt: Q4V8H7 Rat
see all
Anti-WWP1 antibody images:
Western blot - WWP1 antibody (ab43791)
All lanes : Anti-WWP1 antibody (ab43791) at 1 µg/ml
Lane 1 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size : 105 kDa
Observed band size : 105 kDa
Additional bands at : 12.5 kDa,36 kDa,60 kDa. We are unsure as to the identity of these extra bands.
Immunocytochemistry/ Immunofluorescence - WWP1 antibody (ab43791)
ICC/IF image of ab43791 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab43791, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% formaldehyde fixed (10 min) Hek293 and HepG2 cells at 5µg/ml, and in 100% methanol fixed (5 min) HeLa, Hek293, HepG2 and MCF7 cells at 5µg/ml.
References for Anti-WWP1 antibody (ab43791)
This product has been referenced in:
- Anindya R et al. Damage-induced ubiquitylation of human RNA polymerase II by the ubiquitin ligase Nedd4, but not Cockayne syndrome proteins or BRCA1. Mol Cell 28:386-97 (2007).Read more (PubMed: 17996703) »
See 1 publication for this product
Publishing research using ab43791? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"