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Product name
Anti-Werner's syndrome helicase WRN antibody
See all Werner's syndrome helicase WRN products (11) ...
Description
Rabbit polyclonal to Werner's syndrome helicase WRN
Specificity
This antibody is specific for Werner's syndrome helicase. In Western blot the antibody detects a band at ~160 kDa representing Werner's syndrome helicase, not recognised in human fibroblast WRN knock-out cells. Works well in immunofluoresence on WRN +/+ cells and is negative on WRN -/- cells.
Tested applications
ELISA, ICC, ICC/IF, WBmore details
Cross reactivity
Reacts with
Human
Positive control
WRN +/+ cells
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
serum with 0.05% sodium azide
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab200 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at an assay dependent dilution. PubMed: 17118963
ICC: Use at an assay dependent dilution.
ICC/IF: Use at an assay dependent concentration.
WB: 1/2000. Predicted molecular weight: 162 kDa.
Target
Function
Multifunctional enzyme that has both magnesium and ATP-dependent DNA-helicase activity and 3'->5' exonuclease activity towards double-stranded DNA with a 5'-overhang. Has no nuclease activity towards single-stranded DNA or blunt-ended double-stranded DNA. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Important for genomic integrity. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A.
Involvement in disease
Defects in WRN are a cause of Werner syndrome (WRN) [MIM:277700]. WRN is a rare autosomal recessive progeroid syndrome characterized by the premature onset of multiple age-related disorders, including atherosclerosis, cancer, non-insulin-dependent diabetes mellitus, ocular cataracts and osteoporosis. The major cause of death, at a median age of 47, is myocardial infarction. Currently all known WS mutations produces prematurely terminated proteins.
Defects in WRN may be a cause of colorectal cancer (CRC) [MIM:114500].
Sequence similarities
Belongs to the helicase family. RecQ subfamily.
Contains 1 3'-5' exonuclease domain.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 1 HRDC domain.
Post-translational
modifications
Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localization
Nucleus > nucleolus. Nucleus.
Target information above from: UniProt accessionQ14191
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- RecQ-like type 3 antibody
- DKFZp686C2056 antibody
- DNA helicase antibody
see all
Database links
- Entrez Gene: 7486 Human
- Omim: 604611 Human
- SwissProt: Q14191 Human
- Unigene: 632050 Human
Anti-Werner's syndrome helicase WRN antibody images:
Immunocytochemistry/ Immunofluorescence - Werner's syndrome helicase WRN antibody (ab200)
ab200 (1/1000) staining Werner's syndrome helicase WRN in HeLa cells (green). Cells were fixed in methanol, permeabilized with 0.5% Triton X100/ PBS and counterstained with DAPI in order to highlight the nucleus (red). Please refer to abreview for further experimental details.
This image is part of an abreview kindly submitted by Dr. Kirk McManus
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Werner's syndrome helicase WRN antibody (ab200)
ab200 (4µg/ml) staining Werner's syndrome helicase WRN in human colon using an automated system (DAKO Autostainer Plus). Using this protocol there is nuclear and some cytoplasmic staining.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH 6.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
References for Anti-Werner's syndrome helicase WRN antibody (ab200)
This product has been referenced in:
- Sakamoto S et al. Werner helicase relocates into nuclear foci in response to DNA damaging agents and co-localizes with RPA and Rad51. Genes Cells 6:421-30 (2001).Read more (PubMed: 11380620) »
- Rusin M et al. Resveratrol induces senescence-like growth inhibition of U-2 OS cells associated with the instability of telomeric DNA and upregulation of BRCA1. Mech Ageing Dev 130:528-37 (2009). ICC/IF; Human.Read more (PubMed: 19559722) »
See all 12 publications for this product
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"