Anti-Wnt1 antibody [10C8] (ab91191)

Overview

• Product nameAnti-Wnt1 antibody [10C8]See all Wnt1 primary antibodies ...
• Description
  Mouse monoclonal [10C8] to Wnt1
• Tested applicationsWB, ELISA, ICC/IF, IHC-P more details
• Species reactivity
  Reacts with: Mouse, Human
  
• Immunogen

  Purified recombinant fragment of Wnt1 expressed in E. coli.

• Positive controlWB: NIH3T3, 3T3L1 and Hela cell lysate ICC/IF: HeLa, 3T3L1 and HepG2 cells This antibody gave a positive result in IHC in the following FFPE tissue: Human normal skeletal muscle.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.03% Sodium Azide
  Constituents: Ascites
• Concentration information loading...
• PurityAscites
• Clonality Monoclonal
• Clone number10C8
• IsotypeIgG1
• Research Areas
  • Stem Cells
  • Signaling Pathways
  • Wnt
  • Surface Molecules

  • Stem Cells
  • Signaling Pathways
  • Wnt
  • Secreted

  • Cancer
  • Oncoproteins/suppressors
  • Tumor suppressors
  • PTC & Wnt pathway

Applications

Target

• FunctionLigand for members of the frizzled family of seven transmembrane receptors. In some developmental processes, is also a ligand for the coreceptor RYK, thus triggering Wnt signaling. Probable developmental protein. May be a signaling molecule important in CNS development. Is likely to signal over only few cell diameters.
• Sequence similaritiesBelongs to the Wnt family.
• Cellular localizationSecreted > extracellular space > extracellular matrix.

Target information above from: UniProt accession P04628 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 7471 Human
  • Entrez Gene: 22408 Mouse
  • Omim: 164820 Human
  • SwissProt: P04628 Human
  • SwissProt: P04426 Mouse
  • Unigene: 248164 Human
  • Unigene: 1123 Mouse

• Alternative names
  INT1 antibodyProto oncogene protein Wnt 1 antibodyProto-oncogene Int-1 homolog antibody

  Proto-oncogene Wnt-1 antibodyWingless type MMTV integration site family member 1 antibodyWnt 1 antibodywnt1 antibodyWNT1_HUMAN antibody

  see all

Anti-Wnt1 antibody [10C8] images

• 

  Western blot - Wnt1 antibody [10C8] (ab91191)
  All lanes : Anti-Wnt1 antibody [10C8] (ab91191) at 1/500 dilution
  
  Lane 1 : NIH3T3 cell lysate
  Lane 2 : 3T3L1 cell lysate
  Lane 3 : HeLa cell lysate
  
  
  Predicted band size : 41 kDa
  
• 

  Immunocytochemistry/ Immunofluorescence - Wnt1 antibody [10C8] (ab91191)
  ab91191, staining Wnt1 in Hela (left) and 3T3L1 (right) cells by Confocal Immunofluorescence (green).
  Red: Actin filaments that have been labeled with DY-554 phalloidin.
  Blue: DRAQ5 fluorescent DNA dye.
• 

  Immunocytochemistry/ Immunofluorescence - Wnt1 antibody [10C8] (ab91191)
  ab91191 staining Wnt1 in HepG2 cells by Confocal Immunofluorescence (green).
  Red: Actin filaments that have been labeled with DY-554 phalloidin.
  Blue: DRAQ5 fluorescent DNA dye.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt1 antibody [10C8] (ab91191)

  IHC image of Wnt1 staining in Human normal skeletal muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab91191, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.