Anti-Wnt1 antibody [10C8] (ab91191)

Overview

  • Product nameAnti-Wnt1 antibody [10C8]See all Wnt1 primary antibodies ...
  • Description
    Mouse monoclonal [10C8] to Wnt1
  • Tested applicationsWB, ELISA, ICC/IF, IHC-P more details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Purified recombinant fragment of Wnt1 expressed in E. coli.

  • Positive control
    • WB: NIH3T3, 3T3L1 and Hela cell lysate ICC/IF: HeLa, 3T3L1 and HepG2 cells This antibody gave a positive result in IHC in the following FFPE tissue: Human normal skeletal muscle.

Properties

Applications

Our Abpromise guarantee covers the use of ab91191 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB 1/500 - 1/2000. Predicted molecular weight: 41 kDa.
ELISA 1/10000.
ICC/IF Use at an assay dependent dilution.
IHC-P Use a concentration of 5 µg/ml.

Target

  • FunctionLigand for members of the frizzled family of seven transmembrane receptors. In some developmental processes, is also a ligand for the coreceptor RYK, thus triggering Wnt signaling. Probable developmental protein. May be a signaling molecule important in CNS development. Is likely to signal over only few cell diameters.
  • Sequence similaritiesBelongs to the Wnt family.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Target information above from: UniProt accession P04628 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • INT1 antibody
    • Proto oncogene protein Wnt 1 antibody
    • Proto-oncogene Int-1 homolog antibody
    • Proto-oncogene Wnt-1 antibody
    • Wingless type MMTV integration site family member 1 antibody
    • Wnt 1 antibody
    • wnt1 antibody
    • WNT1_HUMAN antibody
    see all

Anti-Wnt1 antibody [10C8] images

  • All lanes : Anti-Wnt1 antibody [10C8] (ab91191) at 1/500 dilution

    Lane 1 : NIH3T3 cell lysate
    Lane 2 : 3T3L1 cell lysate
    Lane 3 : HeLa cell lysate


    Predicted band size : 41 kDa
  • ab91191, staining Wnt1 in Hela (left) and 3T3L1 (right) cells by Confocal Immunofluorescence (green).
    Red: Actin filaments that have been labeled with DY-554 phalloidin.
    Blue: DRAQ5 fluorescent DNA dye.
  • ab91191 staining Wnt1 in HepG2 cells by Confocal Immunofluorescence (green).
    Red: Actin filaments that have been labeled with DY-554 phalloidin.
    Blue: DRAQ5 fluorescent DNA dye.
  • IHC image of Wnt1 staining in Human normal skeletal muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab91191, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-Wnt1 antibody [10C8] (ab91191)

ab91191 has not yet been referenced specifically in any publications.

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