Anti-Wnt1 antibody (Biotin) (ab84243)

Overview

  • Product nameAnti-Wnt1 antibody (Biotin)See all Wnt1 primary antibodies ...
  • Description
    Rabbit polyclonal to Wnt1 (Biotin)
  • ConjugationBiotin
  • Tested applicationsELISA, Sandwich ELISA, WB more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Highly pure (>98%) recombinant Humna Wnt1.

Properties

Applications

Our Abpromise guarantee covers the use of ab84243 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ELISA
Sandwich ELISA
WB
  • Application notesELISA: Use at a concentration of 0.25-1 µg/ml. Detect at least 0.2 – 0.4 ng/well of recombinant Human Wnt1.
    sELISA: Use at a concentration of 0.25-1 µg/ml. Detect at least 0.2 – 0.4 ng/well of recombinant Human Wnt1
    WB: Use at a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 41 kDa.
    The detection limit for recombinant Human Wnt1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionLigand for members of the frizzled family of seven transmembrane receptors. In some developmental processes, is also a ligand for the coreceptor RYK, thus triggering Wnt signaling. Probable developmental protein. May be a signaling molecule important in CNS development. Is likely to signal over only few cell diameters.
    • Sequence similaritiesBelongs to the Wnt family.
    • Cellular localizationSecreted > extracellular space > extracellular matrix.
    • Target information above from: UniProt accession P04628 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
    • Alternative names
      • INT1 antibody
      • Proto oncogene protein Wnt 1 antibody
      • Proto-oncogene Int-1 homolog antibody
      • Proto-oncogene Wnt-1 antibody
      • Wingless type MMTV integration site family member 1 antibody
      • Wnt 1 antibody
      • wnt1 antibody
      • WNT1_HUMAN antibody
      see all

    References for Anti-Wnt1 antibody (Biotin) (ab84243)

    ab84243 has not yet been referenced specifically in any publications.

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    We have validated ab109822 for use with the biotinylated ab84243 and will guarantee these antibodies for use as an sELISA pair. For the positive control, I would recommend using the full length protein, such as ab...

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