You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Anti-Wnt1 antibody (Biotin) (ab84243)

Submit an Abreview Q&A (1)

Overview

  • Product nameAnti-Wnt1 antibody (Biotin)See all Wnt1 primary antibodies ...
  • Description
    Rabbit polyclonal to Wnt1 (Biotin)
  • ConjugationBiotin
  • Tested applicationsELISA, Sandwich ELISA, WB more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Highly pure (>98%) recombinant Humna Wnt1.

Properties

Applications

Our Abpromise guarantee covers the use of ab84243 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ELISA
Sandwich ELISA
WB
  • Application notesELISA: Use at a concentration of 0.25-1 µg/ml. Detect at least 0.2 – 0.4 ng/well of recombinant Human Wnt1.
    sELISA: Use at a concentration of 0.25-1 µg/ml. Detect at least 0.2 – 0.4 ng/well of recombinant Human Wnt1
    WB: Use at a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 41 kDa.
    The detection limit for recombinant Human Wnt1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • FunctionLigand for members of the frizzled family of seven transmembrane receptors. In some developmental processes, is also a ligand for the coreceptor RYK, thus triggering Wnt signaling. Probable developmental protein. May be a signaling molecule important in CNS development. Is likely to signal over only few cell diameters.
    • Sequence similaritiesBelongs to the Wnt family.
    • Cellular localizationSecreted > extracellular space > extracellular matrix.

      • Target information above from: UniProt accession P04628 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
      • Alternative names
          INT1 antibodyProto oncogene protein Wnt 1 antibodyProto-oncogene Int-1 homolog antibody
          Proto-oncogene Wnt-1 antibodyWingless type MMTV integration site family member 1 antibodyWnt 1 antibodywnt1 antibodyWNT1_HUMAN antibody
        see all

      References for Anti-Wnt1 antibody (Biotin) (ab84243)

      ab84243 has not yet been referenced specifically in any publications.

      Publishing research using ab84243 ? Please let us know so that we can cite the reference in this datasheet

      Product Wall

      Submit an Abreview for ab84243 Submit a Question for ab84243

      Displaying 1 - 1 of 1 results for Abreviews and Q&A

      10
      Votes: Highest First

      I have some questions: Do Anti-Wnt1 antibody (Biotin) (ab84243) and Anti-Wnt1 antibody (ab109822) react with different epitops? In other words, can these antibodies be used together for sandwich ELISA? If they can't; can you suggest a wnt-1 antibody pair ...

      Read More

      Thank you for contacting us.

      We have validated ab109822 for use with the biotinylated ab84243 and will guarantee these antibodies for use as an sELISA pair. For the positive control, I would recommend using the full length protein, such as ab840...

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"