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Overview

  • Product nameAnti-Wnt1 antibodySee all Wnt1 primary antibodies ...
  • Description
    Rabbit polyclonal to Wnt1
  • Tested applicationsIndirect ELISA, Sandwich ELISA, WB more details
  • Species reactivity
    Reacts with: Recombinant Fragment
    Predicted to work with: Human
  • Immunogen

    Highly pure (>98%) recombinant Human Wnt1

Properties

Applications

Our Abpromise guarantee covers the use of ab109822 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Indirect ELISA I-ELISA: Use a concentration of 0.5 - 2 µg/ml. ab109822 in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human Wnt1.
Sandwich ELISA sELISA: Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to Wnt1 (Biotin) (ab84243). ab109822 in conjunction with ab84243 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human Wnt1.
WB WB: Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 41 kDa. The detection limit for recombinant Human Wnt1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Target

  • FunctionLigand for members of the frizzled family of seven transmembrane receptors. In some developmental processes, is also a ligand for the coreceptor RYK, thus triggering Wnt signaling. Probable developmental protein. May be a signaling molecule important in CNS development. Is likely to signal over only few cell diameters.
  • Sequence similaritiesBelongs to the Wnt family.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.

    • Target information above from: UniProt accession P04628 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        INT1 antibodyProto oncogene protein Wnt 1 antibodyProto-oncogene Int-1 homolog antibody
        Proto-oncogene Wnt-1 antibodyWingless type MMTV integration site family member 1 antibodyWnt 1 antibodywnt1 antibodyWNT1_HUMAN antibody
      see all

    References for Anti-Wnt1 antibody (ab109822)

    ab109822 has not yet been referenced specifically in any publications.

    Publishing research using ab109822 ? Please let us know so that we can cite the reference in this datasheet

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    Submit an Abreview for ab109822 Submit a Question for ab109822

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    I have some questions: Do Anti-Wnt1 antibody (Biotin) (ab84243) and Anti-Wnt1 antibody (ab109822) react with different epitops? In other words, can these antibodies be used together for sandwich ELISA? If they can't; can you suggest a wnt-1 antibody pair ...

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    Thank you for contacting us.

    We have validated ab109822 for use with the biotinylated ab84243 and will guarantee these antibodies for use as an sELISA pair. For the positive control, I would recommend using the full length protein, such as ab840...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"