Overview

  • Product nameAnti-Wnt1 antibodySee all Wnt1 primary antibodies ...
  • Description
    Rabbit polyclonal to Wnt1
  • Tested applicationsIndirect ELISA, Sandwich ELISA, WBmore details
  • Species reactivity
    Reacts with: Recombinant Fragment
    Predicted to work with: Human
  • Immunogen

    Highly pure (>98%) recombinant Human Wnt1

Properties

Applications

Our Abpromise guarantee covers the use of ab109822 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Indirect ELISA Use a concentration of 0.5 - 2 µg/ml. ab109822 in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human Wnt1.
Sandwich ELISA Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to Wnt1 (Biotin) (ab84243). ab109822 in conjunction with ab84243 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human Wnt1.
WB Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 41 kDa. The detection limit for recombinant Human Wnt1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Target

  • FunctionLigand for members of the frizzled family of seven transmembrane receptors. In some developmental processes, is also a ligand for the coreceptor RYK, thus triggering Wnt signaling. Probable developmental protein. May be a signaling molecule important in CNS development. Is likely to signal over only few cell diameters.
  • Sequence similaritiesBelongs to the Wnt family.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • BMND16 antibody
    • INT1 antibody
    • OI15 antibody
    • oncogene Int1 antibody
    • Proto oncogene protein Wnt 1 antibody
    • Proto-oncogene Int-1 homolog antibody
    • Proto-oncogene Wnt-1 antibody
    • Wingless type MMTV integration site family member 1 antibody
    • wingless-type MMTV integration site family, member 1 (oncogene INT1) antibody
    • Wnt 1 antibody
    • wnt1 antibody
    • WNT1_HUMAN antibody
    see all

References for Anti-Wnt1 antibody (ab109822)

ab109822 has not yet been referenced specifically in any publications.

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We have validated ab109822 for use with the biotinylated ab84243 and will guarantee these antibodies for use as an sELISA pair. For the positive control, I would recommend using the full length protein, such as ab...

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