Anti-Wnt1 antibody (ab15251)

Overview

• Product nameAnti-Wnt1 antibodySee all Wnt1 primary antibodies ...
• Description
  Rabbit polyclonal to Wnt1
• Tested applicationsELISA, IHC-P, IHC-Fr, WB more details
• Species reactivity
  Reacts with: Mouse, Human
  Predicted to work with: Rat
• Immunogen

  Synthetic peptide (unfortunately, the amino acid sequence is considered to be commercially sensitive) (Human) (N terminal).

• Positive controlBreast carcinoma. NIH/3T3 cell lysate.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.1% Sodium Azide
  Constituents: 1% BSA, 10mM PBS, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Stem Cells
  • Signaling Pathways
  • Wnt
  • Secreted

  • Stem Cells
  • Signaling Pathways
  • Wnt
  • Surface Molecules

  • Cancer
  • Oncoproteins/suppressors
  • Tumor suppressors
  • PTC & Wnt pathway

Applications

Target

• FunctionLigand for members of the frizzled family of seven transmembrane receptors. In some developmental processes, is also a ligand for the coreceptor RYK, thus triggering Wnt signaling. Probable developmental protein. May be a signaling molecule important in CNS development. Is likely to signal over only few cell diameters.
• Sequence similaritiesBelongs to the Wnt family.
• Cellular localizationSecreted > extracellular space > extracellular matrix.

Target information above from: UniProt accession P04628 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 7471 Human
  • Entrez Gene: 22408 Mouse
  • Entrez Gene: 24881 Rat
  • Omim: 164820 Human
  • SwissProt: P04628 Human
  • SwissProt: P04426 Mouse
  • Unigene: 248164 Human
  • Unigene: 1123 Mouse

• Alternative names
  INT 1 antibodyINT1 antibodyMurine mammary tumor virus integration site 1 antibody

  Oncogene INT1 antibodyProto oncogene protein Wnt 1 antibodyProto-oncogene Int-1 homolog antibodyProto-oncogene Wnt-1 antibodyWingless type MMTV integration site family member 1 antibodyWnt 1 antibodywnt1 antibodyWnt1 proto oncogene protein precursor antibodyWNT1_HUMAN antibody

  see all

Anti-Wnt1 antibody images

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Wnt1 antibody (ab15251)

  ab15251 staining Wnt1 in breast carcinoma by Immunohistochemistry (FFPE-sections).

• 

  Immunocytochemistry/ Immunofluorescence - Wnt1 antibody (ab15251)

  ICC/IF image of ab15251 stained mouse 3T3 cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab15251, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iT^TM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

• 

  Immunohistochemistry (Formalin-fixed paraffin-embedded sections) - Wnt1 antibody (ab15251)
  Breast carcinoma cells stained with ab15251 (Immunohistochemistry, paraffin sections);
• 

  Western blot - Wnt1 antibody (ab15251)
  Anti-Wnt1 antibody (ab15251) at 1/25 dilution + NIH3T3 cell lysate
  
  Predicted band size : 41 kDa
  Observed band size : 41 kDa