Overview

  • Product nameAnti-Wnt1 antibodySee all Wnt1 primary antibodies ...
  • Description
    Rabbit polyclonal to Wnt1
  • Tested applicationsELISA, IHC-P, IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide (unfortunately, the amino acid sequence is considered to be commercially sensitive) (Human) (N terminal).

  • Positive control
    • Breast carcinoma. NIH/3T3 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab15251 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
IHC-P 1/100.
IHC-Fr Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Predicted molecular weight: 41 kDa.

Target

  • FunctionLigand for members of the frizzled family of seven transmembrane receptors. In some developmental processes, is also a ligand for the coreceptor RYK, thus triggering Wnt signaling. Probable developmental protein. May be a signaling molecule important in CNS development. Is likely to signal over only few cell diameters.
  • Sequence similaritiesBelongs to the Wnt family.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • BMND16 antibody
    • INT 1 antibody
    • INT1 antibody
    • Murine mammary tumor virus integration site 1 antibody
    • OI15 antibody
    • Oncogene INT1 antibody
    • Proto oncogene protein Wnt 1 antibody
    • Proto-oncogene Int-1 homolog antibody
    • Proto-oncogene Wnt-1 antibody
    • Wingless type MMTV integration site family member 1 antibody
    • wingless-type MMTV integration site family, member 1 (oncogene INT1) antibody
    • Wnt 1 antibody
    • wnt1 antibody
    • Wnt1 proto oncogene protein precursor antibody
    • WNT1_HUMAN antibody
    see all

Anti-Wnt1 antibody images

  • ab15251 staining Wnt1 in breast carcinoma by Immunohistochemistry (FFPE-sections).

  • ICC/IF image of ab15251 stained mouse 3T3 cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab15251, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

  • Breast carcinoma cells stained with ab15251 (Immunohistochemistry, paraffin sections);
  • Anti-Wnt1 antibody (ab15251) at 1/25 dilution + NIH3T3 cell lysate

    Predicted band size : 41 kDa
    Observed band size : 41 kDa

References for Anti-Wnt1 antibody (ab15251)

This product has been referenced in:
  • Moreno-Moya JM  et al. The transcriptomic and proteomic effects of ectopic overexpression of miR-30d in human endometrial epithelial cells. Mol Hum Reprod N/A:N/A (2014). Human . Read more (PubMed: 24489115) »
  • Serralbo O & Marcelle C Migrating cells mediate long-range WNT signaling. Development 141:2057-63 (2014). Read more (PubMed: 24803654) »

See all 13 Publications for this product

Product Wall

Application ELISA
Sample Human Recombinant protein (CDK8/CycC)
Specification CDK8/CycC
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 0.1% · Temperature: 22°C
Type Direct
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Submitted Mar 14 2013

Thank you for contacting us.

It may be, as we discussed, that the PFA fixation of the the frozen tissue is the reason the immunostain is not working. I am attaching three papers that deal with antigen retrieval for IHC of frozen tissue. Two ...

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Application Western blot
Loading amount 35 µg
Sample Mouse Cell lysate - whole cell (primary mouse pancreatic cancer cell lines)
Specification primary mouse pancreatic cancer cell lines
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Submitted Dec 15 2010

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (PanIN lesions Pancreas)
Specification PanIN lesions Pancreas
Fixative Acetone
Permeabilization Yes - PBS + Triton 0.025%
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Submitted Dec 06 2010

Application Western blot
Sample Human Cell lysate - whole cell (CaPan1 and Panc1)
Loading amount 35 µg
Specification CaPan1 and Panc1
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Submitted Dec 04 2010

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (pancreatic tumor)
Specification pancreatic tumor
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization Yes - PBST
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
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Submitted Oct 04 2010

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Pancreatic tumor)
Specification Pancreatic tumor
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization Yes - PBST
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
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Submitted Oct 04 2010

Application Western blot
Sample Mouse Cell lysate - whole cell (Podocytes)
Loading amount 30 µg
Specification Podocytes
Treatment 5ng/ml TGF-beta 5min to 4hr
Gel Running Conditions Reduced Denaturing (85 gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Feb 24 2009

Thank you for your enquiry. The immunogen sequence used to raise this product is highly similar to the zebra fish, exhibiting a difference of 2 out of 17aa. If you have any further questions then please get back in touch with me.

Thanks again for your email. No, the cells were not transfected with Wnt1. The lysate was used to detect the endogenous protein. If you have any additional questions, just let us know!

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"