Overview

  • Product nameAnti-Wnt1 antibody
    See all Wnt1 primary antibodies
  • Description
    Rabbit polyclonal to Wnt1
  • Specificityab62703 detects endogenous levels of total Wnt1 protein.
  • Tested applicationsELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide derived from an internal region of human Wnt1.

  • Positive control
    • Human breast carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab62703 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000.
IHC-P 1/50 - 1/100.

Target

  • FunctionLigand for members of the frizzled family of seven transmembrane receptors. In some developmental processes, is also a ligand for the coreceptor RYK, thus triggering Wnt signaling. Probable developmental protein. May be a signaling molecule important in CNS development. Is likely to signal over only few cell diameters.
  • Sequence similaritiesBelongs to the Wnt family.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • BMND16 antibody
    • INT 1 antibody
    • INT1 antibody
    • Murine mammary tumor virus integration site 1 antibody
    • OI15 antibody
    • Oncogene INT1 antibody
    • Proto oncogene protein Wnt 1 antibody
    • Proto-oncogene Int-1 homolog antibody
    • Proto-oncogene Wnt-1 antibody
    • Wingless type MMTV integration site family member 1 antibody
    • wingless-type MMTV integration site family, member 1 (oncogene INT1) antibody
    • Wnt 1 antibody
    • wnt1 antibody
    • Wnt1 proto oncogene protein precursor antibody
    • WNT1_HUMAN antibody
    see all

Anti-Wnt1 antibody images

  • ab62703 at 1/50 - 1/100 dilution staining Wnt1 in human breast carcinoma by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.

References for Anti-Wnt1 antibody (ab62703)

ab62703 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (Breast Cancer Cells (MDA-MB-231, MCF-7, T-47D))
Loading amount 15 µg
Specification Breast Cancer Cells (MDA-MB-231, MCF-7, T-47D)
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted Apr 29 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"