Anti-Wnt1 antibody (ab85060)

Overview

• Product nameAnti-Wnt1 antibodySee all Wnt1 primary antibodies ...
• Description
  Rabbit polyclonal to Wnt1
• Tested applicationsIHC-P, ICC/IF, WB more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human Wnt1.

  Read Abcam's proprietary immunogen policy

  (Peptide available as ab95923.)

• Positive controlThis antibody gave a positive signal in the following human tissue lysates: brain; spinal cord as well as both Mouse and Rat Brain tissue lysates.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 1% BSA, PBS, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Stem Cells
  • Signaling Pathways
  • Wnt
  • Surface Molecules

  • Stem Cells
  • Signaling Pathways
  • Wnt
  • Secreted

  • Cancer
  • Oncoproteins/suppressors
  • Tumor suppressors
  • PTC & Wnt pathway

Applications

Target

• FunctionLigand for members of the frizzled family of seven transmembrane receptors. In some developmental processes, is also a ligand for the coreceptor RYK, thus triggering Wnt signaling. Probable developmental protein. May be a signaling molecule important in CNS development. Is likely to signal over only few cell diameters.
• Sequence similaritiesBelongs to the Wnt family.
• Cellular localizationSecreted > extracellular space > extracellular matrix.

Target information above from: UniProt accession P04628 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 7471 Human
  • Entrez Gene: 22408 Mouse
  • Entrez Gene: 24881 Rat
  • Omim: 164820 Human
  • SwissProt: P04628 Human
  • SwissProt: P04426 Mouse
  • Unigene: 248164 Human
  • Unigene: 1123 Mouse

• Alternative names
  INT1 antibodyProto oncogene protein Wnt 1 antibodyProto-oncogene Int-1 homolog antibody

  Proto-oncogene Wnt-1 antibodyWingless type MMTV integration site family member 1 antibodyWnt 1 antibodywnt1 antibodyWNT1_HUMAN antibody

  see all

Anti-Wnt1 antibody images

• 

  Western blot - Wnt1 antibody (ab85060)
  All lanes : Anti-Wnt1 antibody (ab85060) at 1 µg/ml
  
  Lane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)
  Lane 2 : Spinal Cord (Human) Tissue Lysate - adult normal tissue (ab29188)
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Predicted band size : 41 kDa
  Observed band size : 47 kDa (why is the actual band size different from the predicted?)
  
  
  Exposure time : 5 minutes
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Wnt1 antibody (ab85060)
  IHC image of Wnt1 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab85060, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
• 

  Immunocytochemistry/ Immunofluorescence - Wnt1 antibody (ab85060)
  ICC/IF image of ab85060 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85060, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) HeLa, Hek293 and HepG2 cells at 1µg/ml
• 

  Immunocytochemistry/ Immunofluorescence - Anti-Wnt1 antibody (ab85060)
  ICC/IF image of ab85060 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85060, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  
  
• 

  Western blot - Anti-Wnt1 antibody (ab85060)
  All lanes : Anti-Wnt1 antibody (ab85060) at 1 µg/ml
  
  Lane 1 : Brain (Mouse) Tissue Lysate
  Lane 2 : Brain (Rat) Tissue Lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Predicted band size : 41 kDa
  Observed band size : 41 kDa
  Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.
  
  Exposure time : 2 minutes