Overview

  • Product nameAnti-Wnt10b antibody [5A7]See all Wnt10b primary antibodies ...
  • Description
    Mouse monoclonal [5A7] to Wnt10b
  • Tested applicationsICC/IF, WB, ELISA, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Purified recombinant fragment of Human Wnt10b expressed in E. Coli.

  • Positive control
    • HeLa cell lysate. PANC-1 cells. Human normal stomach, normal liver, normal kidney and rectum cancer tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab91201 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution.
WB 1/500 - 1/2000. Predicted molecular weight: 43 kDa.
ELISA 1/10000.
IHC-P 1/200 - 1/1000.
IHC-Fr 1/200 - 1/1000.

Target

  • FunctionLigand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.
  • Tissue specificityDetected in most adult tissues. Highest levels were found in heart and skeletal muscle. Low levels are found in brain.
  • Involvement in diseaseDefects in WNT10B are the cause of split-hand/foot malformation type 6 (SHFM6) [MIM:225300]. SHFM is a limb malformation involving the central rays of the autopod and presenting with syndactyly, median clefts of the hands and feet, and aplasia and/or hypoplasia of the phalanges, metacarpals, and metatarsals. SHFM6 is a autosomal recessive disorder.
  • Sequence similaritiesBelongs to the Wnt family.
  • Developmental stageInfant brain has higher levels of WNT10B than adult brain.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • Protein Wnt-10b antibody
    • Protein Wnt-12 antibody
    • SHFM6 antibody
    • wingless type MMTV integration site family, member 10B antibody
    • WN10B_HUMAN antibody
    • WNT 10B protein antibody
    • WNT 12 antibody
    • Wnt10b antibody
    • wnt12 antibody
    see all

Anti-Wnt10b antibody [5A7] images

  • All lanes : Anti-Wnt10b antibody [5A7] (ab91201) at 1/500 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : HeLa cell lysate (GAPDH antibody, 37kDa)


    Predicted band size : 43 kDa
  • ab91201 at 1/200 dilution staining Wnt10b in paraffin-embedded human normal stomach (A), normal liver (B), normal kidney (C) and rectum cancer tissues (D) by immunohistochemistry.
  • ab91201 staining Wnt10b in PANC-1 cells by immunocytochemistry (Green). Actin filaments labeled with Alexa Fluor-555 phalloidin (Red). DRAQ5 fluorescent DNA dye (Blue).

References for Anti-Wnt10b antibody [5A7] (ab91201)

This product has been referenced in:
  • Wend P  et al. WNT10B/ß-catenin signalling induces HMGA2 and proliferation in metastatic triple-negative breast cancer. EMBO Mol Med : (2013). IHC-P ; Human . Read more (PubMed: 23307470) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"