Anti-Wnt10b antibody [5A7] (ab91201)

Overview

• Product nameAnti-Wnt10b antibody [5A7]See all Wnt10b primary antibodies ...
• Description
  Mouse monoclonal [5A7] to Wnt10b
• Tested applicationsICC/IF, WB, ELISA, IHC-P, IHC-Fr more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Purified recombinant fragment of Human Wnt10b expressed in E. Coli.

• Positive controlHeLa cell lysate. PANC-1 cells. Human normal stomach, normal liver, normal kidney and rectum cancer tissues.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Store at -20ºC.
• Storage bufferPreservative: 0.03% Sodium Azide
  Constituents: Ascites
• Concentration information loading...
• PurityAscites
• Clonality Monoclonal
• Clone number5A7
• IsotypeIgG1
• Research Areas
  • Metabolism
  • Types of disease
  • Cancer

  • Stem Cells
  • Signaling Pathways
  • Wnt
  • Secreted

  • Neuroscience
  • Neurology process
  • Metabolism

Applications

Target

• FunctionLigand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.
• Tissue specificityDetected in most adult tissues. Highest levels were found in heart and skeletal muscle. Low levels are found in brain.
• Involvement in diseaseDefects in WNT10B are the cause of split-hand/foot malformation type 6 (SHFM6) [MIM:225300]. SHFM is a limb malformation involving the central rays of the autopod and presenting with syndactyly, median clefts of the hands and feet, and aplasia and/or hypoplasia of the phalanges, metacarpals, and metatarsals. SHFM6 is a autosomal recessive disorder.
• Sequence similaritiesBelongs to the Wnt family.
• Developmental stageInfant brain has higher levels of WNT10B than adult brain.
• Cellular localizationSecreted > extracellular space > extracellular matrix.

Target information above from: UniProt accession O00744 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 7480 Human
  • Omim: 601906 Human
  • SwissProt: O00744 Human
  • Unigene: 91985 Human

• Alternative names
  Protein Wnt-10b antibodyProtein Wnt-12 antibodySHFM6 antibody

  wingless type MMTV integration site family, member 10B antibodyWN10B_HUMAN antibodyWnt10b antibodywnt12 antibody

  see all

Anti-Wnt10b antibody [5A7] images

• 

  Western blot - Wnt10b antibody [5A7] (ab91201)
  All lanes : Anti-Wnt10b antibody [5A7] (ab91201) at 1/500 dilution
  
  Lane 1 : HeLa cell lysate
  Lane 2 : HeLa cell lysate (GAPDH antibody, 37kDa)
  
  
  Predicted band size : 43 kDa
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Wnt10b antibody [5A7] (ab91201)
  ab91201 at 1/200 dilution staining Wnt10b in paraffin-embedded human normal stomach (A), normal liver (B), normal kidney (C) and rectum cancer tissues (D) by immunohistochemistry.
• 

  Immunocytochemistry/ Immunofluorescence - Wnt10b antibody [5A7] (ab91201)
  ab91201 staining Wnt10b in PANC-1 cells by immunocytochemistry (Green). Actin filaments labeled with Alexa Fluor-555 phalloidin (Red). DRAQ5 fluorescent DNA dye (Blue).