Anti-Wnt10b antibody (ab66721)

Overview

• Product nameAnti-Wnt10b antibodySee all Wnt10b primary antibodies ...
• Description
  Rabbit polyclonal to Wnt10b
• Tested applicationsICC/IF, WB more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse, Rat, Horse, Pig, Chimpanzee
• Immunogen

  Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Wnt10b.

  Read Abcam's proprietary immunogen policy

  (Peptide available as ab66720.)

• Positive controlThis antibody gave a positive signal in Human MCF7 Whole Cell Lysate

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 1% BSA, PBS, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Metabolism
  • Types of disease
  • Cancer

  • Stem Cells
  • Signaling Pathways
  • Wnt
  • Secreted

  • Neuroscience
  • Neurology process
  • Metabolism

Applications

Target

• FunctionLigand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.
• Tissue specificityDetected in most adult tissues. Highest levels were found in heart and skeletal muscle. Low levels are found in brain.
• Involvement in diseaseDefects in WNT10B are the cause of split-hand/foot malformation type 6 (SHFM6) [MIM:225300]. SHFM is a limb malformation involving the central rays of the autopod and presenting with syndactyly, median clefts of the hands and feet, and aplasia and/or hypoplasia of the phalanges, metacarpals, and metatarsals. SHFM6 is a autosomal recessive disorder.
• Sequence similaritiesBelongs to the Wnt family.
• Developmental stageInfant brain has higher levels of WNT10B than adult brain.
• Cellular localizationSecreted > extracellular space > extracellular matrix.

Target information above from: UniProt accession O00744 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 7480 Human
  • Entrez Gene: 22410 Mouse
  • Entrez Gene: 315294 Rat
  • Omim: 601906 Human
  • SwissProt: O00744 Human
  • SwissProt: P48614 Mouse
  • Unigene: 91985 Human
  • Unigene: 4709 Mouse

• Alternative names
  Protein Wnt-10b antibodyProtein Wnt-12 antibodySHFM6 antibody

  wingless type MMTV integration site family, member 10B antibodyWN10B_HUMAN antibodyWnt10b antibodywnt12 antibody

  see all

Anti-Wnt10b antibody images

• 

  Western blot - Anti-Wnt10b antibody (ab66721)
  Anti-Wnt10b antibody (ab66721) at 1 µg/ml + MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg
  
  Secondary
  Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
  
  Performed under reducing conditions.
  
  Predicted band size : 43 kDa
  Observed band size : 43 kDa
  
• 

  Immunocytochemistry/ Immunofluorescence - Wnt10b antibody (ab66721)
  ICC/IF image of ab66721 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab66721, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive result in 100% methanol fixed (5 min) HeLa, and MCF-7 cells at 1µg/ml, and in 4% pfa fixed (10 min) HeLa, HepG2, MCF-7 cells at 1µg/ml.