Overview

  • Product nameAnti-Wnt10b antibody
    See all Wnt10b primary antibodies
  • Description
    Rabbit polyclonal to Wnt10b
  • Tested applicationsICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Pig, Chimpanzee
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Wnt10b.

    (Peptide available as ab66720.)

  • Positive control
    • This antibody gave a positive signal in Human MCF7 Whole Cell Lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab66721 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).Can be blocked with Human Wnt10b peptide (ab66720).

Target

  • FunctionLigand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.
  • Tissue specificityDetected in most adult tissues. Highest levels were found in heart and skeletal muscle. Low levels are found in brain.
  • Involvement in diseaseDefects in WNT10B are the cause of split-hand/foot malformation type 6 (SHFM6) [MIM:225300]. SHFM is a limb malformation involving the central rays of the autopod and presenting with syndactyly, median clefts of the hands and feet, and aplasia and/or hypoplasia of the phalanges, metacarpals, and metatarsals. SHFM6 is a autosomal recessive disorder.
  • Sequence similaritiesBelongs to the Wnt family.
  • Developmental stageInfant brain has higher levels of WNT10B than adult brain.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • Protein Wnt-10b antibody
    • Protein Wnt-12 antibody
    • SHFM6 antibody
    • wingless type MMTV integration site family, member 10B antibody
    • WN10B_HUMAN antibody
    • WNT 10B protein antibody
    • WNT 12 antibody
    • Wnt10b antibody
    • wnt12 antibody
    see all

Anti-Wnt10b antibody images

  • Anti-Wnt10b antibody (ab66721) at 1 µg/ml + MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 43 kDa
    Observed band size : 43 kDa
  • ICC/IF image of ab66721 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab66721, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive result in 100% methanol fixed (5 min) HeLa, and MCF-7 cells at 1µg/ml, and in 4% pfa fixed (10 min) HeLa, HepG2, MCF-7 cells at 1µg/ml.

References for Anti-Wnt10b antibody (ab66721)

ab66721 has not yet been referenced specifically in any publications.

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