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Overview

  • Product nameAnti-Wnt10b antibodySee all Wnt10b primary antibodies ...
  • Description
    Rabbit polyclonal to Wnt10b
  • Specificityab70816 only recognizes the longer isoform of Wnt10b and will not cross-react with Wnt10a.
  • Tested applicationsICC/IF, IHC-P, WB more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    15 amino acid peptide from near the center of human Wnt10b (NP_003385).

  • Positive controlHuman skeletal muscle tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab70816 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC/IF ICC/IF: Use a concentration of 5 µg/ml.
IHC-P IHC-P: Use a concentration of 2.5 µg/ml.
WB WB: Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 43 kDa).

Target

  • FunctionLigand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.
  • Tissue specificityDetected in most adult tissues. Highest levels were found in heart and skeletal muscle. Low levels are found in brain.
  • Involvement in diseaseDefects in WNT10B are the cause of split-hand/foot malformation type 6 (SHFM6) [MIM:225300]. SHFM is a limb malformation involving the central rays of the autopod and presenting with syndactyly, median clefts of the hands and feet, and aplasia and/or hypoplasia of the phalanges, metacarpals, and metatarsals. SHFM6 is a autosomal recessive disorder.
  • Sequence similaritiesBelongs to the Wnt family.
  • Developmental stageInfant brain has higher levels of WNT10B than adult brain.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.

    • Target information above from: UniProt accession O00744 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        Protein Wnt-10b antibodyProtein Wnt-12 antibodySHFM6 antibody
        wingless type MMTV integration site family, member 10B antibodyWN10B_HUMAN antibodyWnt10b antibodywnt12 antibody
      see all

    Anti-Wnt10b antibody images

    • Western blot - Wnt10b antibody (ab70816)
      Western blot - Wnt10b antibody (ab70816)
      Lane 1 : Anti-Wnt10b antibody (ab70816) at 2 µg/ml
      Lane 2 : Anti-Wnt10b antibody (ab70816) at 4 µg/ml

      Lane 1 : Human skeletal muscle tissue lysate
      Lane 2 : Human skeletal muscle tissue lysate

      Lysates/proteins at 15 µg per lane.


      Predicted band size : 43 kDa
      Observed band size : 47 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 93 kDa. We are unsure as to the identity of these extra bands.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Wnt10b antibody (ab70816)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Wnt10b antibody (ab70816)

      ab70816 at 2.5 µ/ml staining Wnt10 in human skeletal muscle tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).

    • Immunocytochemistry/ Immunofluorescence-Wnt10b antibody(ab70816)
      Immunocytochemistry/ Immunofluorescence-Wnt10b antibody(ab70816)
      ICC/IF image of ab70816 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70816, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    References for Anti-Wnt10b antibody (ab70816)

    This product has been referenced in:
    • Fei Y  et al. Fibroblast growth factor 2 stimulation of osteoblast differentiation and bone formation is mediated by modulation of the wnt signaling pathway. J Biol Chem 286:40575-83 (2011). WB ; Mouse . Read more (PubMed: 21987573) »

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    Western blot abreview for Anti-Wnt10b antibody

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    Abreviews
    Application Western blot
    Sample Mouse Cell lysate - whole cell (3T3-L1 preadipocytes)
    Loading amount 16.6 µg
    Specification 3T3-L1 preadipocytes
    Treatment With vehicle (DMSO) or Tunicamycin (10 µg/ml for 6h or 24h)
    Gel Running Conditions Reduced Denaturing (Invitrogen NuPAGE 4-14% Bis-Tris Gel)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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    Submitted May 11 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"