|
|
|
|
|
Product Name
|
XAB1 antibody
|
| |
See all XAB1 antibodies (3)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rabbit polyclonal to XAB1
|
|
Immunogen
|
Synthetic peptide: DTDDIDHRVTEESHEEP, corresponding to amino acids 339-355 of Human XAB1 conjugated to KLH via a N terminal added cysteine residue.
BLAST 'DTDDIDHRVTEESHEEP' with  or 
|
|
Reacts with
(species key)
|
Hu, Ms
|
|
Tested applications
(see key)
|
IP, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
IP: Use at a concentration of 5 - 10 µg/ml lysate
WB: Use at a concentration of 0.25 - 0.5 µg/ml. Predicted molecular weight: 42 kDa.
Staining of the MBDin/XAB1 band in Western blotting is specifically inhibited by the
immunizing peptide.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Positive control
(see definition)
|
HEK-293T cell lysates
|
|
Cellular localization
|
Cytoplasmic
|
|
Research areas
|
Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Nucl. Excision Repair
Chromatin and Nuclear Signaling >> DNA methylation >> Methylated DNA
|
|
Relevance
|
The DNA methylation system is composed of methyl-CpG-binding proteins, as well as of DNA cytosine methyl transferases. Five methyl-CpG binding proteins were isolated: MeCP2, MBD1, MBD2, MBD3 and MBD4. MBD2 consists of two isoforms, MBD2a and MBD2b, which are generated from a single gene. MBD2a is a component of MeCP1, a large corepressor complex that represses transcription from densely methylated genes. Components of MeCP1 include MBD2, Mi-2, MTA2, MBD3 and HDAC1/2. MIZF and MBDin were isolated as proteins interacting with MBD2. MBDin, in turn, is identical to XPA binding protein 1 (XAB1). At the N terminus, the protein contains the consensus sequence for a GTP binding site. At the C terminus, the protein is characterized by an acidic domain containing a cluster of acidic amino acid residues. The transcriptional repression by MBD2 at methylated promoters is relieved by MBdin. XAB1 is expressed ubiquitously, and may be involved in nuclear localization of XPA.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Rabbit
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
Immunogen affinity purified
|
|
Storage buffer
|
Preservative: 15mM Sodium Azide
Constituents: 1% BSA, 0.01M PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
|
|
Form
|
Liquid
|
|
Concentration
|
0.600 mg/ml
|
|
Storage instructions
|
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this XAB1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
| |
|
Search PubMed (MEDLINE) for references to XAB1
|
| |
XAB1 antibody - more information
|
Customer reviews (feedback) regarding XAB1 antibody |
Customer FAQs regarding XAB1 antibody |
Protocols for XAB1 antibody |
Price and availability of products related to XAB1 antibody |
| |
XAB1 antibody - related products:
|
|
|
| |
XAB1 antibody - other tools:
|
Contact Abcam with a Technical Enquiry about XAB1 antibody |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
